National Citrus Engineering Research Center, Citrus Research Institute, Southwest University, Chongqing, 400712, China.
Academy of Agricultural Sciences, Southwest University, Chongqing, 400715, China.
Arch Virol. 2021 Jul;166(7):2055-2058. doi: 10.1007/s00705-021-05082-2. Epub 2021 May 5.
A novel positive-stranded RNA virus provisionally named "citrus virus C" (CVC) was discovered in citrus trees displaying mottling symptoms. Its genome comprises 7,215 nucleotides (nt), excluding the 3' poly(A) tail, and contains two open reading frames (ORFs) that encode a replication-associated polyprotein (RP) and a putative coat protein (CP). The CVC genome contains a 16-nt 'marafibox', which is highly conserved in most viruses belonging to the genus Marafivirus of the same family. Sequence analysis suggested that the virus is most closely related to grapevine Red Globe virus (GRGV), which is yet to be officially classified in the family Tymoviridae. The sequence identities between CVC and GRGV in the whole genome (50.7%, nt) and CP (49.4% for amino acid, and 53.9% for nt) are lower than the thresholds (80% in the genome and 90% in the CP) for species demarcation in the family. Therefore, it is legitimate to propose that CVC is a member of new species in the family Tymoviridae.
一种新型正链 RNA 病毒被暂时命名为“柑橘病毒 C”（CVC），它在出现斑驳症状的柑橘树上被发现。其基因组由 7215 个核苷酸（nt）组成，不包括 3' 多聚（A）尾，包含两个开放阅读框（ORFs），分别编码复制相关多蛋白（RP）和假定的外壳蛋白（CP）。CVC 基因组包含一个 16 个核苷酸的“marafibox”，在同一科的 Marafivirus 属的大多数病毒中高度保守。序列分析表明，该病毒与尚未在 Tymoviridae 科正式分类的葡萄红环球病毒（GRGV）最为密切相关。CVC 和 GRGV 在整个基因组（nt 50.7%）和 CP（氨基酸 49.4%，nt 53.9%）中的序列同一性均低于科内物种划分的阈值（基因组 80%，CP 90%）。因此，有理由提出 CVC 是 Tymoviridae 科新种的成员。
