Istituto Agronomico Mediterraneo di Bari, Via Ceglie 9, 70010 Valenzano, Bari, Italy.
Virus Res. 2011 Nov;161(2):198-202. doi: 10.1016/j.virusres.2011.07.022. Epub 2011 Aug 4.
The complete nucleotide sequence and the genome organization were determined of a novel virus, tentatively named Fig fleck-associated virus (FFkaV). The viral genome is a positive-sense, single-stranded RNA 7046 nucleotides in size excluding the 3'-terminal poly(A) tract, and comprising two open reading frames. ORF1 encodes a polypeptide of 2161 amino acids (p240), which contains the signatures of replication-associated proteins and the coat protein cistron (p24) at its 3' end. ORF2 codes for a 461 amino acid protein (p50) identified as a putative movement proteins (MP). In phylogenetic trees constructed with sequences of the putative polymerase and CP proteins FFkaV consistently groups with members of the genus Maculavirus, family Tymoviridae. However, the genome organization diverges from that of the two completely sequenced maculaviruses, Grapevine fleck virus (GFkV) and Bombix mori Macula-like virus (BmMLV), as it exhibits a structure resembling that of Maize rayado fino virus (MRFV), the type species of the genus Marafivirus and of Olive latent virus 3 (OLV-3), an unclassified virus in the family Tymoviridae. FFkaV was found in field-grown figs from six Mediterranean countries with an incidence ranging from 15% to 25%.
一种新型病毒的完整核苷酸序列和基因组结构已被确定，暂命名为无花果斑伴随病毒（FFkaV）。该病毒的基因组是一条正链、单链 RNA，大小为 7046 个核苷酸，不包括 3'末端的 poly(A) 尾，包含两个开放阅读框。ORF1 编码一个由 2161 个氨基酸组成的多肽（p240），其 3' 端含有复制相关蛋白和外壳蛋白结构域（p24）的特征。ORF2 编码一个 461 个氨基酸的蛋白（p50），被鉴定为一个假定的运动蛋白（MP）。在基于推定聚合酶和 CP 蛋白序列构建的系统发育树中，FFkaV 始终与 Maculavirus 属的成员聚在一起，属于 Tymoviridae 科。然而，基因组结构与两种完全测序的 Maculavirus 不同，即葡萄斑病毒（GFkV）和 BmMLV，因为它表现出类似于 Maize rayado fino virus（MRFV）的结构，MRFV 是 Marafivirus 属和 Olive latent virus 3（OLV-3）的模式种，OLV-3 是 Tymoviridae 科中的一种未分类病毒。FFkaV 存在于来自六个地中海国家的田间生长的无花果中，发病率在 15%至 25%之间。
