Itoh F, Horie T, Awazu S
Department of Biopharmaceutics, Tokyo College of Pharmacy, Japan.
Arch Biochem Biophys. 1988 Jul;264(1):184-91. doi: 10.1016/0003-9861(88)90584-x.
The fluorescence emitted from rat liver microsomal membranes which had undergone enzymatic and nonenzymatic lipid peroxidation was detected directly. This fluorescence produced in peroxidized membranes increased progressively with peroxidation reaction time, and the fluorescent substances produced were retained in the membranes without being released into the aqueous phase. Extracts of the peroxidized membranes with organic solvents (chloroform/methanol) emitted fluorescence which was also dependent on the peroxidation reaction time. The generation profiles of fluorescence emitted from both the peroxidized membranes and their extracted membrane lipids differed essentially from that of thiobarbituric acid-reactive substances which reached a plateau at a relatively early stage of peroxidation reaction. These results indicate that lipid peroxidation induces stepwise chemical and physical changes in membranes and that the fluorescence from peroxidized membranes will be useful in studying such changes occurring in biological membranes.
直接检测了经历酶促和非酶促脂质过氧化作用的大鼠肝微粒体膜发出的荧光。过氧化膜中产生的这种荧光随着过氧化反应时间的延长而逐渐增加，并且产生的荧光物质保留在膜中，而不会释放到水相中。用过氧化膜的有机溶剂（氯仿/甲醇）提取物发出的荧光也取决于过氧化反应时间。过氧化膜及其提取的膜脂发出的荧光生成曲线与硫代巴比妥酸反应性物质的生成曲线基本不同，后者在过氧化反应的相对早期阶段达到平稳期。这些结果表明，脂质过氧化作用会在膜中引起逐步的化学和物理变化，并且过氧化膜发出的荧光将有助于研究生物膜中发生的此类变化。
