Wang Zhe, Li Xu-Ling, Hong Kin-Fong, Zhao Ting-Ting, Dong Rui-Xue, Wang Wei-Ming, Li Yun-Tong, Zhang Gui-Lin, Lin Jing, Gui Ding-Kun, Xu You-Hua
Faculty of Chinese Medicine, State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Taipa, Macao, China.
School of Pharmacy, State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Taipa, Macao, China.
Evid Based Complement Alternat Med. 2021 Apr 12;2021:6675567. doi: 10.1155/2021/6675567. eCollection 2021.
is one of the common traditional Chinese medicines used to treat diabetes. However, the underlying mechanism is not fully understood. Flavones are a class of active components that have been reported to exert various activities. Existing evidence suggests that flavones from may be pivotal in modulating progression of diabetes. In this study, total flavones from (TFA) were studied to observe its effects on metabolism of bile acids both and . C57BL/6J mice were treated with STZ and high-fat feeding to construct diabetic model, and HepG2 cell line was applied to investigate the influence of TFA on liver cells. We found a serious disturbance of bile acids and lipid metabolism in diabetic mice, and oral administration or cell incubation with TFA significantly reduced the production of total cholesterol (TCHO), total triglyceride, glutamic oxalacetic transaminase (AST), glutamic-pyruvic transaminase (ALT), and low-density lipoprotein (LDL-C), while it increased the level of high-density lipoprotein (HDL-C). The expression of glucose transporter 2 (GLUT2) and cholesterol 7-hydroxylase (CYP7A1) was significantly upregulated on TFA treatment, and FXR and TGR5 play pivotal role in modulating bile acid and lipid metabolism. This study supplied a novel understanding towards the mechanism of on controlling diabetes.
是用于治疗糖尿病的常见中药之一。然而，其潜在机制尚未完全明确。黄酮类化合物是一类已被报道具有多种活性的活性成分。现有证据表明，来自[具体来源未提及]的黄酮类化合物可能在调节糖尿病进展中起关键作用。在本研究中，对[具体来源未提及]的总黄酮（TFA）进行了研究，以观察其对[具体体内外模型未明确]胆汁酸代谢的影响。用链脲佐菌素（STZ）和高脂喂养处理C57BL/6J小鼠以构建糖尿病模型，并应用HepG2细胞系研究TFA对肝细胞的影响。我们发现糖尿病小鼠胆汁酸和脂质代谢严重紊乱，口服TFA或用TFA进行细胞孵育可显著降低总胆固醇（TCHO）、总甘油三酯、谷氨酸草酰乙酸转氨酶（AST）、谷氨酸丙酮酸转氨酶（ALT）和低密度脂蛋白（LDL-C）的产生，同时提高高密度脂蛋白（HDL-C）水平。TFA处理后，葡萄糖转运蛋白2（GLUT2）和胆固醇7α-羟化酶（CYP7A1）的表达显著上调，法尼醇X受体（FXR）和G蛋白偶联胆汁酸受体5（TGR5）在调节胆汁酸和脂质代谢中起关键作用。本研究为[具体中药未明确]控制糖尿病的机制提供了新的认识。
