利什曼原虫细胞的鸟枪法脂质组学分析

Shotgun Lipidomic Analysis of Leishmania Cells.

作者信息

Zhang Kai, Hsu Fong-Fu

机构信息

Department of Biological Sciences, Texas Tech University, Lubbock, TX, USA.

Mass Spectrometry Resource, Division of Endocrinology, Metabolism, and Lipid Research, Department of Medicine, Washington University School of Medicine, St. Louis, MO, USA.

出版信息

Methods Mol Biol. 2021;2306:215-225. doi: 10.1007/978-1-0716-1410-5_14.

DOI:10.1007/978-1-0716-1410-5_14

PMID:33954949

Abstract

To understand the interplay of lipids between Leishmania promastigotes, amastigotes, and vertebrate host cells, a robust method for cultivating Leishmania parasites, lipid extraction, and shotgun lipidomic analysis with loop injection is described. This book chapter provides the step-by-step workflow to guide readers from sample preparation to the global lipid analysis by multiple stage mass spectrometry with high resolution and tandem quadrupole mass spectrometric approaches toward studying the metabolomic roles that lipids may play in Leishmania parasite infections.

摘要

为了解利什曼原虫前鞭毛体、无鞭毛体和脊椎动物宿主细胞之间脂质的相互作用，本文描述了一种用于培养利什曼原虫寄生虫、脂质提取以及采用环进样进行鸟枪法脂质组分析的可靠方法。本章提供了详细的工作流程，以指导读者从样品制备到通过高分辨率多级质谱和串联四极杆质谱方法进行全局脂质分析，从而研究脂质在利什曼原虫感染中可能发挥的代谢组学作用。