通过微阵列分析检测克罗恩病患者结肠活检组织中环状 RNA 的表达谱。
Expression profiles of circular RNAs in colon biopsies from Crohn's disease patients by microarray analysis.
机构信息
Basic Medical Laboratory, Institute of Clinical Laboratory Science, Jinling Hospital, Nanjing University School of Medicine, Nanjing, China.
Department of Gastroenterology, Drum Tower Hospital, Nanjing University School of Medicine, Nanjing, China.
出版信息
J Clin Lab Anal. 2021 Jun;35(6):e23788. doi: 10.1002/jcla.23788. Epub 2021 May 6.
BACKGROUND
Circular RNAs (circRNAs) are involved in various diseases and serve as biomarkers. The present study aimed to investigate unique expression profiles of circRNAs in colon tissues of Crohn's disease (CD) and search novel biomarkers for the diagnosis.
METHODS
Differentially expressed (DE) circRNAs in biopsies from four CD patients, four ulcerative colitis (UC) patients, and four healthy controls (HC) were screened by microarray. Hsa_circ_0062142 and hsa_circ_0001666 were verified in another expanded validation cohort. Bioinformatics analysis was applied to predict the function of two DE circRNAs. Receiver operating characteristic (ROC) curves were constructed to evaluate the diagnostic value of CD.
RESULTS
The top 10 upregulated circRNAs in CD compared with HC were hsa_circ_0000691, hsa_circ_0001666, hsa_circ_0004183, hsa_circ_0009024, hsa_circ RNA_405324, hsa_circ_0002003, hsa_circ_0085323, hsa_circ_0040994, hsa_circ_0062142, and hsa_circ_0048148; the top 10 downregulated circRNAs were hsa_circ_0049356, hsa_circ RNA_405443, hsa_circ RNA_403556, hsa_circ_0092328, hsa_circ_0003979, hsa_circ_0074491, hsa_circ_0023461, hsa_circ RNA_406237, hsa_circ_0034044, and hsa_circ RNA_400564 (fold change in descending order). The expression levels of hsa_circ_0001666 and hsa_circ_0062142 in CD were significantly higher than those in UC and HC (p < 0.01). ROC curves suggested the favorable diagnostic value of hsa_circ_0062142 and hsa_circ_0001666 (AUC = 0.803 and 0.858, respectively, p < 0.01). In silico analysis indicated that these circRNAs may be involved in the progress of CD.
CONCLUSION
Hsa_circ_0062142 and hsa_circ_0001666 may play critical roles in the pathogenesis and serve as potential biomarkers of CD.
背景
环状 RNA（circRNAs）参与多种疾病，并可作为生物标志物。本研究旨在探讨克罗恩病（CD）结肠组织中circRNAs 的独特表达谱，并寻找用于诊断的新型生物标志物。
方法
通过微阵列筛选来自 4 名 CD 患者、4 名溃疡性结肠炎（UC）患者和 4 名健康对照（HC）的活检组织中的差异表达（DE）circRNAs。在另一个扩展验证队列中验证 hsa_circ_0062142 和 hsa_circ_0001666。应用生物信息学分析预测两个 DE circRNAs 的功能。构建受试者工作特征（ROC）曲线以评估 CD 的诊断价值。
结果
与 HC 相比，CD 中上调的前 10 个 circRNAs 为 hsa_circ_0000691、hsa_circ_0001666、hsa_circ_0004183、hsa_circ_0009024、hsa_circ_RNA_405324、hsa_circ_0002003、hsa_circ_0085323、hsa_circ_0040994、hsa_circ_0062142 和 hsa_circ_0048148；下调的前 10 个 circRNAs 为 hsa_circ_0049356、hsa_circ_RNA_405443、hsa_circ_RNA_403556、hsa_circ_0092328、hsa_circ_0003979、hsa_circ_0074491、hsa_circ_0023461、hsa_circ_RNA_406237、hsa_circ_0034044 和 hsa_circ_RNA_400564（按降序排列）。CD 中 hsa_circ_0001666 和 hsa_circ_0062142 的表达水平明显高于 UC 和 HC（p<0.01）。ROC 曲线提示 hsa_circ_0062142 和 hsa_circ_0001666 具有良好的诊断价值（AUC 分别为 0.803 和 0.858，p<0.01）。计算分析表明，这些 circRNAs 可能参与了 CD 的进展。
结论
hsa_circ_0062142 和 hsa_circ_0001666 可能在 CD 的发病机制中发挥关键作用，并可作为 CD 的潜在生物标志物。
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