Kumar A, Rao P, Pattabiraman T N
Department of Biochemistry, Kasturba Medical College, Karnataka, India.
Biochem Med Metab Biol. 1988 Jun;39(3):296-304. doi: 10.1016/0885-4505(88)90089-8.
A new colorimetric method based on the phenol-sulfuric acid reaction is described for the estimation of serum glycated proteins by the differential reduction of free glucose and hexose bound nonenzymatically with 2.0 and 20 mg of NaBH4 in 0.02 ml of serum, respectively, at room temperature for 15 min. The values (microgram hexose/mg protein) in control subjects (n = 60) and diabetics (n = 90) were estimated to be 5.60 +/- 0.85 and 10.8 +/- 1.6, respectively. The increase was highly significant (P less than 0.001) in diabetics. The serum glycated protein levels correlate well with fasting blood sugar values (r = 0.77, P less than 0.001, n = 25). There was also a highly significant correlation between glycated protein level and glycated albumin value in individual serum samples (r = 0.85, P less than 0.001, n = 25). Values of borohydride reducible glyco-groups bound to serum proteins also correlated well with serum glycated protein levels (r = 0.96, p less than 0.001, n = 20) determined by the thiobarbituric acid assay method. The method is found to be simple and rapid, with a coefficient of variations of +/- 3.8%.
本文描述了一种基于苯酚 - 硫酸反应的比色法，用于估算血清糖化蛋白。该方法是通过在室温下分别用2.0毫克和20毫克硼氢化钠对0.02毫升血清中游离葡萄糖和非酶结合的己糖进行差异还原15分钟。对照组（n = 60）和糖尿病患者（n = 90）的测定值（微克己糖/毫克蛋白质）分别为5.60±0.85和10.8±1.6。糖尿病患者的增加非常显著（P < 0.001）。血清糖化蛋白水平与空腹血糖值相关性良好（r = 0.77，P < 0.001，n = 25）。在个体血清样本中，糖化蛋白水平与糖化白蛋白值之间也存在高度显著的相关性（r = 0.85，P < 0.001，n = 25）。与血清蛋白结合的硼氢化物可还原糖基的值与通过硫代巴比妥酸测定法测定的血清糖化蛋白水平也相关性良好（r = 0.96，p < 0.001，n = 20）。该方法简单快速，变异系数为±3.8%。
