Marrelli Mariangela, De Marco Caterina Tecla, Statti Giancarlo, Neag Teodora Alina, Toma Claudia-Crina, Conforti Filomena
Department of Pharmacy, Health and Nutritional Sciences, University of Calabria, Rende, Italy.
Faculty of Pharmacy, Vasile Goldis Western University, Arad, Romania.
Nat Prod Res. 2022 Jun;36(11):2859-2863. doi: 10.1080/14786419.2021.1920018. Epub 2021 May 6.
Aerial parts and roots from three species were extracted with 70% ethanol. The phytochemical composition was investigated using GC-MS and multivariate data analysis. The total phenolic and flavonoid contents were also assessed. The in vitro inhibitory properties were evaluated as nitrite concentration in LPS-stimulated RAW 264.7 macrophage cell line. All the samples induced concentration-dependent inhibitory effects, with aerial parts extract being the most interesting sample (IC50 = 22.08 ± 1.32 µg/mL), even more active than the reference compound indomethacin. A very good activity was also observed for and aerial parts extracts (IC50 = 51.61 ± 3.12 µg/mL and 84.55 ± 3.40 µg/mL). A lesser but noteworthy potential was also demonstrated for the root extracts. The results suggest that hydroalcoholic extracts are able to inhibit nitrite accumulation and may be useful in preventing inflammatory diseases mediated by excessive production of NO.
从三种植物的地上部分和根部用70%乙醇进行提取。使用气相色谱 - 质谱联用仪（GC - MS）和多变量数据分析研究其植物化学成分。还评估了总酚和黄酮含量。以脂多糖（LPS）刺激的RAW 264.7巨噬细胞系中的亚硝酸盐浓度来评估体外抑制特性。所有样品均表现出浓度依赖性抑制作用，其中地上部分提取物是最具吸引力的样品（半数抑制浓度IC50 = 22.08 ± 1.32微克/毫升），甚至比参比化合物吲哚美辛更具活性。对于[此处原文缺失植物名称]地上部分提取物也观察到了非常好的活性（IC50 = 51.61 ± 3.12微克/毫升和84.55 ± 3.40微克/毫升）。根部提取物也显示出较小但值得注意的潜力。结果表明，[此处原文缺失植物名称]水醇提取物能够抑制亚硝酸盐积累，并且可能有助于预防由一氧化氮过度产生介导的炎症性疾病。
