Department of Dermatology, Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou, China.
Department of Immunology, Basic Medical School, Guizhou Medical University, Guiyang, Guizhou, China.
Immun Inflamm Dis. 2021 Sep;9(3):840-850. doi: 10.1002/iid3.438. Epub 2021 May 6.
OPN3 upregulation associated with metastasis was recently described in two subtypes of lung cancers. And OPN3 identified in light-independent functions in epidermal melanocytes has already shown promise. However, in malignant melanocytic tissues, the expression and characterization of OPN3 remain uncharacterized.
We investigated the clinico-histopathologic features in relation to OPN3 expression of acral lentiginous melanoma (ALM), which is a rare cutaneous melanoma subtype and not associated with prior sunlight exposure.
In all, 84 samples of junctional melanocytic nevi (JMN, n = 12), primary ALMs (n = 39) and inguinal lymph node metastasis (ILNM, n = 23) from ALMs were evaluated for the immunohistochemical expression of OPN3. OPN3 messenger RNA and protein level were further determined in melanocytic tumors using quantitative real-time PCR, multiimmunofluorescence and Western blot assays. We also estimated the associations OPN3 expression between clinicopathological features and prognosis.
ILNMs, in contrast to JMN and ALMs, had higher OPN3 expression scores (p < .001) by immunohistochemistry analysis. High OPN3 score was associated with presence of ulceration, increased Breslow depth and Clark level (p = .025, p = .042, and p = .012, respectively). Furthermore, a remarkable difference (p = .037) of patient overall survival was found when comparing the OPN3 expression of immunohistochemical score between equal to or larger than 100 and below 100 groups. Also, Cox regression models showed that high OPN3 scores were associated with worse melanoma survival.
High OPN3 expression is significantly associated with ALMs and metastatic phenotype as well as a poor prognosis.
最近在两种肺癌亚型中描述了 OPN3 的上调与转移相关。并且在光非依赖性功能中鉴定出的 OPN3 在表皮黑素细胞中已经显示出前景。然而，在恶性黑素细胞组织中，OPN3 的表达和特征仍然未知。
我们研究了与肢端黑色素瘤（ALM）相关的 OPN3 表达的临床病理特征，ALM 是一种罕见的皮肤黑色素瘤亚型，与先前的阳光暴露无关。
总共评估了 84 例交界性黑色素细胞痣（JMN，n=12）、原发性 ALM（n=39）和腹股沟淋巴结转移（ILNM，n=23）的 OPN3 免疫组织化学表达。使用定量实时 PCR、多免疫荧光和 Western blot 检测黑色素瘤中 OPN3 信使 RNA 和蛋白水平。我们还估计了 OPN3 表达与临床病理特征和预后之间的相关性。
与 JMN 和 ALM 相比，ILNMs 的 OPN3 表达评分更高（p<0.001）通过免疫组织化学分析。高 OPN3 评分与溃疡存在、Breslow 深度和 Clark 水平增加相关（p=0.025，p=0.042，p=0.012）。此外，当比较免疫组织化学评分等于或大于 100 与小于 100 组之间的 OPN3 表达时，患者总生存存在显著差异（p=0.037）。此外，Cox 回归模型显示，高 OPN3 评分与黑色素瘤生存率较差相关。
高 OPN3 表达与 ALM 及其转移表型以及不良预后显著相关。
