Shandong Provincial Key Laboratory of Biochemical Engineering, College of Marine Science and Biological Engineering, Qingdao University of Science & Technology, Qingdao, Shandong, China.
Qingdao Institute of Agricultural Science Research, Qingdao, Shandong, China.
PLoS One. 2021 May 6;16(5):e0249108. doi: 10.1371/journal.pone.0249108. eCollection 2021.
Cucurbita maxima belong to the genus Cucurbita and are of nutritional and economic importance. Physiological activity, transcriptome, and metabolome analyses of leaf samples from the C. maxima inbreding line IL7 treated at 5 °C and 25 °C were performed. Cold stress resulted in a significant increase in the malondialdehyde content, relative electrical conductivity, soluble protein, sugar content, and catalase activity. A total of 5,553 differentially expressed genes were identified, of which 2,871 were up-regulated and 2,682 down-regulated. In addition, the transcription of differentially expressed genes in the plant hormone signal transduction pathway and transcription factor families of AP2/ERF, bHLH, WRKY, MYB, and HSF was activated. Moreover, 114 differentially expressed metabolites were identified by gas chromatography time-of-flight mass spectrometry, particularly through the analysis of carboxylic acids and derivatives, and organooxygen compounds. The demonstration of a series of potential metabolites and corresponding genes highlighted a comprehensive regulatory mechanism. These findings will provide novel insights into the molecular mechanisms associated with the response to cold stress in C. maxima.
南瓜属于南瓜属，具有重要的营养和经济价值。对在 5°C 和 25°C 下处理的自交系 IL7 的南瓜叶片样本进行了生理活性、转录组和代谢组分析。冷胁迫导致丙二醛含量、相对电导率、可溶性蛋白、糖含量和过氧化氢酶活性显著增加。共鉴定出 5553 个差异表达基因，其中 2871 个上调，2682 个下调。此外，植物激素信号转导途径和 AP2/ERF、bHLH、WRKY、MYB 和 HSF 转录因子家族中差异表达基因的转录被激活。此外，通过气相色谱飞行时间质谱鉴定出 114 种差异表达代谢物，特别是通过对羧酸及其衍生物和含氧化合物的分析。一系列潜在代谢物及其相应基因的证明突出了一个全面的调控机制。这些发现将为南瓜应对冷胁迫的分子机制提供新的见解。
