Hubei Key Laboratory of Natural Medicinal Chemistry and Resource Evaluation, School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, People's Republic of China.
Leibniz Institute for Natural Product Research and Infection Biology - HKI, 07745 Jena, Germany.
J Am Chem Soc. 2021 May 19;143(19):7267-7271. doi: 10.1021/jacs.1c02868. Epub 2021 May 6.
Enzymes that can perform halogenation of aliphatic carbons are of significant interest to the synthetic and biocatalysis communities. Here we describe the characterization of AoiQ, a single-component flavin-dependent halogenase (FDH) that catalyzes dichlorination of 1,3-diketone substrates in the biosynthesis of dichlorodiaporthin. AoiQ represents the first biochemically reconstituted FDH that can halogenate an enolizable sp-hybridized carbon atom.
能够对脂肪族碳原子进行卤化的酶在合成和生物催化领域引起了人们的极大兴趣。在这里，我们描述了 AoiQ 的特征，AoiQ 是一种单组分黄素依赖性卤化酶（FDH），它在二氯二孢素的生物合成中催化 1,3-二酮底物的二氯化。AoiQ 代表了第一个可对烯醇化的 sp 杂化碳原子进行卤化的生物化学重建的 FDH。
