College of Life Science, Northwest University, North Tabai Road, Beilin District, Xi'an, 710069, People's Republic of China.
Key Laboratory of Resource Biology and Modern Biotechnology in Western China of Education Ministry, Northwest University, Xi'an, 710069, People's Republic of China.
Sci Rep. 2021 May 6;11(1):9717. doi: 10.1038/s41598-021-88511-1.
To provide basis for improving the quality of Gentianae macrophyllae Radix and optimising the field processing method. Gentianae macrophyllae Radix samples were collected from Long County. Five main active iridoids were determined by using HPLC. The HPLC fingerprints were measured and analysed by the traditional Chinese medicine chromatographic fingerprint similarity evaluation system. Water, total ash, acid insoluble ash and ethanol extract contents were determined in accordance with Chinese Pharmacopoeia (2015). Colour of the surface and powdered samples were determined by using a colorimeter. Data were analysed by SPSS11.0. Notable differences were found among samples from different sweating and drying processes, in spite of the relatively consistent overall chemical characteristics. Sweating and drying methods influenced the contents of some active ingredients and colour very significantly; moreover, they also showed significant effects on the water, ash, and ethanol extract contents. The necessity of sweating treatment in the field processing of Gentianae macrophyllae Radix may be insufficient. Drying in the shade and oven drying are more profitable for preserving the active constituents. Gentianae macrophyllae Radix should be dried directly without sweating, and drying in the shade and oven drying should be adopted preferentially to improve the quality.
为提高秦艽药材质量和优化产地加工方法提供依据。采集龙州县秦艽药材样品，采用高效液相色谱法测定 5 种主要活性环烯醚萜类成分的含量，应用中药色谱指纹图谱相似度评价系统对其指纹图谱进行测定和分析。按《中国药典》（2015 年版）规定的方法测定水分、总灰分、酸不溶性灰分和醇溶性浸出物的含量，用测色色差计测定表面及粉末颜色。采用 SPSS11.0 软件对数据进行分析。结果表明，不同发汗和干燥工艺的秦艽样品尽管整体化学成分比较一致，但仍存在显著差异。发汗和干燥方法对部分活性成分和颜色有非常显著的影响，对水分、灰分和醇溶性浸出物含量也有显著影响。秦艽产地加工过程中发汗处理的必要性可能不足。阴干和烘干更有利于保存有效成分。秦艽药材应直接干燥，避免发汗，优先采用阴干和烘干，以提高质量。
