Mobasherizadeh Sina, Shojaei Hasan, Azadi Davood, Havaei Seyed Asghar, Ataei Behrooz, Khorvash Farzin
Nosocomial Infection Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.
Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Adv Biomed Res. 2021 Jan 27;10:3. doi: 10.4103/abr.abr_139_20. eCollection 2021.
Methicillin-resistant (MRSA) has become a considerable public health concern in the entire world due to the rapid spread of this bacterium in human community; also the epidemiology of MRSA has changed, as the isolation of MRSA strains from healthy and non-healthy patients. Therefore, the objective of this study is to determine the genetic diversity and antibiotic resistance profile of community-acquired (CA)-MRSA nasal carriage in the Iranian samples.
A total of 25 CA-MRSA were isolated from the anterior nares of 410 healthy preschool children. All MRSA isolates were characterized by the detection of the toxic shock syndrome toxin-1 (TSST-1) and typed by γ-hemolysin genes, groups, and staphylococcal protein A () typing. Kirby-Buyer antibiotic susceptibility testing was performed and interpreted as per the standard guidelines.
A total of 25 (6.1%) MRSA isolates were recovered from the anterior nares of 410 preschool children. Sixteen isolates (64%) were positive for the TSST-1 gene. Three specificity groups were determined, as follows: eight (32%) isolates belonged to Group I, five (20%) isolates belonged to Group II, and 12 (48%) isolates belonged to Group III. The repeated profiles of these types of 25 isolates were organized into eight different lineages groups. Five of lineages contained a single strain, three of lineages contained two strains, and three of lineages consisted of more than three strains.
The results of our study show that the rate of MRSA in our region is significantly high. Additionally, type t037 was the predominant type among CA .
耐甲氧西林金黄色葡萄球菌（MRSA）由于在人类群体中的迅速传播，已成为全球范围内相当严重的公共卫生问题；而且MRSA的流行病学也发生了变化，因为从健康和非健康患者中都分离出了MRSA菌株。因此，本研究的目的是确定伊朗样本中社区获得性（CA）-MRSA鼻腔定植的遗传多样性和抗生素耐药性概况。
从410名健康学龄前儿童的前鼻孔中总共分离出25株CA-MRSA。所有MRSA分离株通过检测中毒性休克综合征毒素-1（TSST-1）进行鉴定，并通过γ-溶血素基因、组别和葡萄球菌蛋白A（）分型进行分型。按照标准指南进行Kirby-Buyer抗生素敏感性试验并进行解释。
从410名学龄前儿童的前鼻孔中总共分离出25株（6.1%）MRSA分离株。16株（64%）分离株的TSST-1基因呈阳性。确定了三个特异性组，如下：8株（32%）分离株属于组I，5株（20%）分离株属于组II，12株（48%）分离株属于组III。这25株分离株的这些类型的重复图谱被组织成八个不同的谱系组。五个谱系包含单个菌株，三个谱系包含两个菌株，三个谱系由三个以上菌株组成。
我们的研究结果表明，我们地区的MRSA感染率显著较高。此外，t037型是CA中占主导地位的类型。
