Taylor S R, Blatt J, Costantino J P, Roederer M, Murphy R F
Pathology Department, Children's Hospital, Pittsburgh, PA 15213.
Cancer. 1988 Aug 15;62(4):749-54. doi: 10.1002/1097-0142(19880815)62:4<749::aid-cncr2820620418>3.0.co;2-w.
Retrospective quantitative DNA analysis was done on 147 samples from 89 patients with neuroblastoma and ganglioneuroma using flow cytometry. In the neuroblastoma patients, nuclear DNA content was found to be a stable tumor marker irrespective of site (primary versus metastatic) and despite changes with time in tumor progression, maturation, or therapy. The occurrence of DNA aneuploidy, which was detected in 60% of the neuroblastoma patients, paralleled other favorable indicators and was highly associated with survival (P less than 0.001). Of clinical stage, age, primary site, sex, and DNA content, only stage and DNA content correlated with survival. Those patients with favorable stage and DNA aneuploidy had higher survival rates. Further, favorable stage and the presence of DNA aneuploidy were independent prognostic indicators. Abnormal DNA content was also detected in samples from ganglioneuromas in which significant numbers of ganglion cell nuclei were recovered. These results indicate a striking difference between neuroblastoma and adult tumors in which DNA aneuploidy is generally a poor prognostic sign and provide a molecular link between ganglioneuromas and their malignant counterparts.
使用流式细胞术对89例神经母细胞瘤和神经节神经瘤患者的147份样本进行了回顾性定量DNA分析。在神经母细胞瘤患者中，无论肿瘤部位（原发灶与转移灶）如何，也不管肿瘤进展、成熟或治疗过程中随时间发生的变化，核DNA含量都是一个稳定的肿瘤标志物。在60%的神经母细胞瘤患者中检测到DNA非整倍体，其出现与其他有利指标平行，且与生存率高度相关（P<0.001）。在临床分期、年龄、原发部位、性别和DNA含量中，只有分期和DNA含量与生存率相关。那些分期良好且存在DNA非整倍体的患者生存率更高。此外，良好的分期和DNA非整倍体的存在是独立的预后指标。在回收了大量神经节细胞核的神经节神经瘤样本中也检测到了异常DNA含量。这些结果表明神经母细胞瘤与成人肿瘤之间存在显著差异，在成人肿瘤中DNA非整倍体通常是不良预后标志，并且为神经节神经瘤与其恶性对应物之间提供了分子联系。
