Fluorosis Foundation of India (Camping & Working for JICA Funded 'Fluorosis Mitigation Programme' in Nagaur District, Rajasthan, India), New Delhi, India.
Adv Exp Med Biol. 2021;1306:121-127. doi: 10.1007/978-3-030-63908-2_9.
Analysis of fluoride in body fluids (urine and serum) is essential for fluorosis diagnosis. Although 24-h urine collection is adopted to assess community defluoridation/fluoride supplementation program/research studies, but not feasible for Clinical/Pathological laboratories. Patients are reluctant to bring 24-h urine samples. Hence, spot urine samples are collected in clean, dry polypropylene bottles (not glass) without any preservative and analyzed on the same day by the Ion analyzer (ISE method). Equal volumes of Total Ionic Strength Adjustment Buffer (TISAB) solution are then added with body fluids before analysis and mixed well to eliminate interference from other ions besides pH adjustment and to provide a constant ionic strength. Results are reported as mg of Fluoride/l (ppm). High fluoride level in body fluids is an indication of confirmed cases of fluorosis. Two interventions, e.g. withdrawal of fluoride intake and intake of nutritive diet was introduced for prevention and control of fluorosis. The present study is to provide useful guidelines for monitoring of fluorosis disease in human beings, those who are at the risk of fluoride exposure.
分析体液（尿液和血清）中的氟化物对于氟中毒的诊断至关重要。虽然 24 小时尿液收集被用于评估社区除氟/氟化物补充计划/研究，但在临床/病理实验室中并不实用。患者不愿意带来 24 小时尿液样本。因此，在清洁、干燥的聚丙烯瓶（不是玻璃）中收集单次尿液样本，无需任何防腐剂，并在当天通过离子分析仪（ISE 法）进行分析。在分析前，将等量的总离子强度调节剂（TISAB）溶液与体液混合均匀，以消除除 pH 调整外其他离子的干扰，并提供恒定的离子强度。结果以氟化物毫克/升（ppm）报告。体液中氟化物含量高是氟中毒确诊病例的指征。两种干预措施，如停止摄入氟化物和摄入营养饮食，已被引入用于预防和控制氟中毒。本研究旨在为监测人类氟中毒疾病提供有用的指导，这些疾病可能与氟化物暴露有关。
