Woon Marites T, Pandey Puspa R, Friedrich Ben-Nun Inbar
Cell and Gene Therapy Research and Development, Lonza Inc., Rockville, MD, USA.
Methods Mol Biol. 2022;2436:39-53. doi: 10.1007/7651_2021_396.
Bioreactor technolology enables the expansion of mammalian cells, which can be translated to processes compatible with Current Good Manufacturing Practice (cGMP) regulations. Cells are introduced to the bioreactor vessel, wherein key parameters such as temperature, pH, and oxygen levels are tightly controlled to facilitate growth over time. Here, we describe the microcarrier-based expansion of human pluripotent stem cells in a 3 L stirred tank bioreactor.
生物反应器技术能够实现哺乳动物细胞的扩增，这可以转化为符合现行药品生产质量管理规范（cGMP）法规的工艺。将细胞引入生物反应器容器中，在其中严格控制温度、pH值和氧气水平等关键参数，以促进细胞随时间生长。在此，我们描述了在3升搅拌罐生物反应器中基于微载体的人多能干细胞扩增。
