Branda R F, McCormack J J, Perlmutter C A, Mathews L A, Robison S H
Department of Medicine, University of Vermont, Burlington 05405.
Cancer Res. 1988 Aug 15;48(16):4529-34.
Experiments were designed to measure the effect of folic acid deficiency on a major determinant of cancer lethality, the propensity to form metastases. Murine B16 melanoma cells (F10 strain) were grown in folate-deficient and -supplemented media. After 3 days, cells in the deficient medium had restricted proliferative capacity, low folate levels by bioassay, increased cell volume, abnormal deoxyuridine suppression tests, accumulation of cells in S phase by flow cytometry, and increased numbers of DNA strand breaks. These folate-deficient cells consistently initiated more pulmonary metastases than control cells when injected into host mice. Cell size did not appear to be a major factor in pulmonary metastasis formation. In vitro growth rates and cloning efficiencies were comparable for cells in both types of medium as was subcutaneous growth of tumors. We conclude that folate deficiency increases the metastatic potential of cultured melanoma cells.
实验旨在测定叶酸缺乏对癌症致死率的一个主要决定因素（即形成转移的倾向）的影响。将小鼠B16黑色素瘤细胞（F10株）培养于叶酸缺乏和补充叶酸的培养基中。3天后，缺乏叶酸培养基中的细胞增殖能力受限，通过生物测定法检测叶酸水平较低，细胞体积增大，脱氧尿苷抑制试验异常，通过流式细胞术检测发现细胞在S期积聚，且DNA链断裂数量增加。当将这些缺乏叶酸的细胞注射到宿主小鼠体内时，它们始终比对照细胞引发更多的肺转移。细胞大小似乎不是肺转移形成的主要因素。两种培养基中的细胞体外生长速率和克隆效率相当，肿瘤的皮下生长情况也相当。我们得出结论，叶酸缺乏会增加培养的黑色素瘤细胞的转移潜能。
