Microgenesis Co, Detroit, MI, USA.
ABM Laboratorios Tandil, Buenos Aires, Argentina.
Am J Reprod Immunol. 2021 Oct;86(4):e13438. doi: 10.1111/aji.13438. Epub 2021 May 17.
The aim of this study was to investigate the possible relationship between vaginal/rectal microbiome disbalances and miRNA expression with infertility.
Observational, exploratory, preliminary study. A total of 287 multiple IVF failure infertile patients were recruited. Twenty fertile women, not IVF failure, were recruited as the control group. Swab samples were collected from the vagina and rectum. Microbial composition by NGS and miRNA expression by real-time PCR of vaginal and rectal samples was measured. Immunometabolic markers from blood (insulin, vitamin D, LDL-cholesterol, ANA, TPO, Tg, and ASCA antibodies) and saliva (sIgA) were analyzed.
RESULT(S): Infertile patients showed a lower bacterial richness and increased Firmicutes/Bacteroidetes ratio at rectal level and an increased Lactobacillus brevis/Lactobacillus iners ratio in vaginal samples regarding the fertile group. In the same rectal swab samples, we found that miR-21-5p, which is associated with tight junction disruption and yeast overgrowth, is upregulated and that miR-155-5p, which is associated with inflammation, is overexpressed in the unexplained infertile group (*p < .05). These deregulated miRNAs were also upregulated in the vaginal samples from the same patients (*p < .05).
miRNAs could be potential biomarkers of the inflammatory impact of microbiome disbalances in unexplained infertile women.
本研究旨在探讨阴道/直肠微生物组失衡与 miRNA 表达与不孕之间的可能关系。
观察性、探索性、初步研究。共招募了 287 名多次 IVF 失败的不孕患者。招募了 20 名未进行 IVF 失败的生育力正常的女性作为对照组。采集阴道和直肠拭子样本。通过 NGS 测量阴道和直肠样本的微生物组成，通过实时 PCR 测量 miRNA 表达。分析血液（胰岛素、维生素 D、LDL-胆固醇、ANA、TPO、Tg 和 ASCA 抗体）和唾液（sIgA）中的免疫代谢标志物。
与生育组相比，不孕患者直肠水平的细菌丰富度降低，厚壁菌门/拟杆菌门比值增加，阴道样本中的短双歧杆菌/短乳杆菌比值增加。在相同的直肠拭子样本中，我们发现与紧密连接破坏和酵母过度生长相关的 miR-21-5p 上调，与炎症相关的 miR-155-5p 在不明原因不孕组中过度表达（*p<.05）。来自同一患者的阴道样本中这些失调的 miRNA 也上调（*p<.05）。
miRNA 可能是不明原因不孕妇女微生物组失衡炎症影响的潜在生物标志物。
