Amrita Centre for Nanosciences and Molecular Medicine, Amrita Vishwa Vidyapeetham, Kochi, Kerala 682024, India.
Amrita Centre for Nanosciences and Molecular Medicine, Amrita Vishwa Vidyapeetham, Kochi, Kerala 682024, India.
Int J Biol Macromol. 2021 Jul 31;183:1200-1209. doi: 10.1016/j.ijbiomac.2021.04.181. Epub 2021 May 4.
Promising strategies to stabilize gelatin or collagen include glutaraldehyde-based chemical cross-linking or dehydrothermal treatment at different temperatures (120-180 °C). However, these procedures require 24-48 h for complete cross-linking to occur. The present study aims to evaluate the role of wheat gluten on enhancing thermal cross-linking of silica-nanohydroxyapatite (nanoHA)-gelatin composite scaffolds within a shorter period (2 h). Changes in properties were evaluated by varying the ratio of gelatin and gluten in silica-nanoHA matrix (60 wt% ceramic: 40 wt% polymer). The results showed that the scaffolds cross-linked at 170 °C were stable in phosphate-buffered saline for 21 days. It was crystalline and porous in nature. However, the scaffolds with high weight percentage of wheat gluten were brittle, while those with low gluten degraded fast in vitro. The mesenchymal stem cells could adhere, proliferate and differentiate into osteogenic lineage on wheat gluten-containing scaffolds for 21 days (mainly medium concentration). The scaffold also supported new bone formation in critical-sized rat calvarial defect, showing its osteoconductive and osteointegrative nature. In short, this study showed the potential of wheat gluten on improving thermal cross-linking within a shorter period and its suitability to use as a biomimetic bone graft for bone tissue engineering.
有前途的稳定明胶或胶原蛋白的策略包括戊二醛基化学交联或不同温度（120-180°C）的脱水热处理。然而，这些程序需要 24-48 小时才能完全交联。本研究旨在评估小麦面筋在更短时间（2 小时）内增强硅酸钠纳米羟基磷灰石（nanoHA）-明胶复合支架的热交联作用。通过改变硅酸钠纳米 HA 基质中明胶和面筋的比例（60wt%陶瓷：40wt%聚合物）来评估性能变化。结果表明，在 170°C 交联的支架在磷酸盐缓冲盐溶液中 21 天内稳定。它是结晶和多孔的。然而，含有高重量百分比面筋的支架易碎，而含有低面筋的支架在体外快速降解。间充质干细胞可以在含面筋的支架上粘附、增殖并分化为成骨谱系 21 天（主要是中等浓度）。支架还支持在大鼠临界颅骨缺损中形成新骨，显示其骨传导和骨整合特性。总之，本研究表明了小麦面筋在更短时间内提高热交联的潜力及其作为仿生骨移植物用于骨组织工程的适用性。
