重齿千里光（菊科）精油：化学成分及抗菌活性测试

The essential oil of Senecio graveolens (Compositae): chemical composition and antimicrobial activity tests.

作者信息

Pérez C, Agnese A M, Cabrera J L

机构信息

Cátedra de Farmacología, Facultad de Odontología, Universidad de Buenos Aires, Argentina.

出版信息

J Ethnopharmacol. 1999 Jul;66(1):91-6. doi: 10.1016/s0378-8741(98)00204-9.

DOI:10.1016/s0378-8741(98)00204-9

PMID:10432213

Abstract

The chemical composition of the essential oil obtained from Senecio graveolens (Compositae) was analyzed by GLC-MS and the components identified were: isovaleraldehyde, alpha-pinene, alpha-phellandrene, alpha-terpinene, p-cymene, sabinene, gamma-terpinene, 1-methyl-4-isopropenylbenzene, terpinolene, terpinen-4-ol, piperitenone, alpha- and beta-eudesmol. The investigation by the agar-well diffusion method of the antimicrobial activity of the essential oil proved that it has antibacterial effects on Micrococcus luteus ATCC 9341, oxacillin-sensitive and oxacillin-resistant Staphylococcus aureus, as well as antifungal effects on clinically isolated Candida albicans. The minimal inhibitory concentration (MIC) values for M. luteus, oxacillin-sensitive S. aureus and C. albicans were 8.73, 10.91 and 2.13 x 10(-2) mg/ml, respectively. The antimicrobial activity related to known antibiotics was calculated. These results would be compatible with a potential concentration-dependent selectivity of antifungal effect of S. graveolens essential oil. Minimal bactericidal concentration (MBC) is above 87.3 mg/ml. Thus the MBC:MIC ratio would be clearly higher than 1 (above 8), indicating a bacteriostatic effect of the essential oil.

摘要

采用气相色谱 - 质谱联用（GLC - MS）分析法对从粗齿千里光（菊科）中提取的挥发油化学成分进行了分析，鉴定出的成分有：异戊醛、α - 蒎烯、α - 水芹烯、α - 松油烯、对伞花烃、桧烯、γ - 松油烯、1 - 甲基 - 4 - 异丙烯基苯、萜品油烯、萜品 - 4 - 醇、胡椒酮、α - 和β - 桉叶醇。通过琼脂孔扩散法对挥发油的抗菌活性进行研究，结果表明其对藤黄微球菌ATCC 9341、对苯唑西林敏感和耐药的金黄色葡萄球菌具有抗菌作用，对临床分离的白色念珠菌具有抗真菌作用。藤黄微球菌、对苯唑西林敏感的金黄色葡萄球菌和白色念珠菌的最低抑菌浓度（MIC）值分别为8.73、10.91和2.13×10⁻²mg/ml。计算了与已知抗生素相关的抗菌活性。这些结果与粗齿千里光挥发油抗真菌作用可能存在的浓度依赖性选择性相符。最低杀菌浓度（MBC）高于87.3mg/ml。因此，MBC:MIC比值明显高于1（高于8），表明挥发油具有抑菌作用。