Temasek Life Sciences Laboratory, National University of Singapore, 1 Research Link, 117604, Singapore.
Temasek Life Sciences Laboratory, National University of Singapore, 1 Research Link, 117604, Singapore; Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, 117543, Singapore.
Curr Opin Plant Biol. 2021 Oct;63:102047. doi: 10.1016/j.pbi.2021.102047. Epub 2021 May 6.
RNA modifications constitute an essential layer of gene regulation in living organisms. As the most prevalent internal modification on eukaryotic mRNAs, N-methyladenosine (mA) exists in many plant species and requires the evolutionarily conserved methyltransferases, demethylases, and mA binding proteins for writing, erasing, and reading mA, respectively. In plants, mA affects many aspects of mRNA metabolism, including alternative polyadenylation, secondary structure, translation, and decay, which underlies various plant developmental processes and stress responses. Here, we discuss the recent progress in understanding the roles of mA modification in mRNA metabolism and their mechanistic link with plant development and stress responses. We also highlight some outstanding questions and provide an outlook on future prospects of mA research in plants.
RNA 修饰是生物体内基因调控的一个重要层面。N6-甲基腺苷（m6A）是真核生物 mRNA 上最普遍的内部修饰之一，存在于许多植物物种中，分别需要进化上保守的甲基转移酶、去甲基酶和 m6A 结合蛋白来进行 m6A 的写入、擦除和读取。在植物中，m6A 影响 mRNA 代谢的许多方面，包括可变多聚腺苷酸化、二级结构、翻译和降解，这是各种植物发育过程和应激反应的基础。在这里，我们讨论了在理解 m6A 修饰在 mRNA 代谢中的作用及其与植物发育和应激反应的机制联系方面的最新进展。我们还强调了一些悬而未决的问题，并对植物中 m6A 研究的未来前景进行了展望。
