Chen Xiaoyu, Kang Yu, Li Shun, Yang Bo, Xia Xiaoyan, Wang Zhonghua, Qian Lunwen, Xiong Xinhua, Kang Lei, He Xin
Hunan Agricultural University, Hunan, 410128, China.
Yue Lu Shan Laboratory, Hunan, 410125, China.
BMC Genomics. 2025 May 24;26(1):526. doi: 10.1186/s12864-025-11695-7.
N-methyladenosine (mA) modification involves the addition of a methyl group to the nitrogen atom at position six of adenine in RNA. It is the most prevalent type of dynamic internal RNA methylation modification, plays an important role in plant development and abiotic stress. The mA modification is facilitated by mA writers (mA methyltransferases), mA erasers (mA demethylation enzymes), and mA readers (mA methylated reading proteins).
In order to study the characterization and expression of mA methyltransferases and demethylases in Brassica napus (rapeseed), we used five methyltransferases and two demethylases from Arabidopsis thaliana as reference sequences. A total of 34 methyltransferases and 12 demethylases were identified in B. napus, B. oleracea, and B. rapa. We analyzed the physicochemical properties, gene structures, conserved domains, chromosome localization, and expression pattern across all tissues, as well as the effects of hormone and stress treatments on B. napus. Our findings revealed that the methyltransferase BnaHAKAI was highly expressed during the late stages of seed development. It may be related to the synthesis of oil content and seed size in the later stage of seed growth. In contrast, the demethylase BnaALKBH10B exhibited high expression primarily in the petals, followed by the pods, buds. This expression pattern may be associated with flower development and the timing of flowering. Furthermore, BnaALKBH10B primarily responded to abiotic stresses such as salinity, drought, osmotic, cold, and freezing, as well as to hormones like jasmonic acid and gibberellins. The qRT-PCR results showed that BnaALKBH10B responded to freezing and salt stress.
In summary, a total of 34 methyltransferases and 12 demethylases genes were identified in B. napus, B. oleracea, and B. rapa, and their phylogenetic relationships, structural domains, and expression patterns in tissues and under abiotic stress were comprehensively analyzed. This research will serve as a foundation for future studies on mA in B. napus.
N-甲基腺苷（mA）修饰是指在RNA腺嘌呤的第六位氮原子上添加一个甲基。它是最普遍的动态内部RNA甲基化修饰类型，在植物发育和非生物胁迫中起重要作用。mA修饰由mA写入器（mA甲基转移酶）、mA擦除器（mA去甲基化酶）和mA读取器（mA甲基化阅读蛋白）促成。
为了研究甘蓝型油菜（油菜籽）中mA甲基转移酶和去甲基化酶的特性与表达，我们使用来自拟南芥的5种甲基转移酶和2种去甲基化酶作为参考序列。在甘蓝型油菜、甘蓝和白菜型油菜中总共鉴定出34种甲基转移酶和12种去甲基化酶。我们分析了它们的理化性质、基因结构、保守结构域、染色体定位、在所有组织中的表达模式，以及激素和胁迫处理对甘蓝型油菜的影响。我们的研究结果表明，甲基转移酶BnaHAKAI在种子发育后期高表达。它可能与种子生长后期的含油量和种子大小的合成有关。相比之下，去甲基化酶BnaALKBH10B主要在花瓣中高表达，其次是豆荚、芽。这种表达模式可能与花的发育和开花时间有关。此外，BnaALKBH10B主要对盐度、干旱、渗透、寒冷和冷冻等非生物胁迫以及茉莉酸和赤霉素等激素作出反应。qRT-PCR结果表明BnaALKBH10B对冷冻和盐胁迫有反应。
总之，在甘蓝型油菜、甘蓝和白菜型油菜中总共鉴定出了34个甲基转移酶基因和12个去甲基化酶基因，并对它们的系统发育关系、结构域以及在组织中和非生物胁迫下的表达模式进行了全面分析。本研究将为未来对甘蓝型油菜中mA的研究奠定基础。
