Gouda Mostafa, Huang Zhenxiong, Liu Yufei, He Yong, Li Xiaoli
College of Biosystems Engineering and Food Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, China; Department of Nutrition & Food Science, National Research Centre, Dokki, Giza, Egypt.
College of Biosystems Engineering and Food Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, China.
Bioresour Technol. 2021 Aug;334:125232. doi: 10.1016/j.biortech.2021.125232. Epub 2021 May 1.
This study aimed to evaluate the functionality of bioactive terpenes on Spirulina (Arthrospira platensis; AP) and Chlorella (Chlorella vulgaris; CV) biomasses. The two microalgae species were treated with 0.01%, 0.05%, and 0.1% of thymol (THY), trans-cinnamaldehyde (TC), menthol (MEN), and vanillin (VAN). Raman micro-spectroscopy (RMS) was correlated with other physicochemical methods to confirm their functional mechanisms. In results, THY (0.1%) decreased (P < 0.05) RMS intensity at 1196 cm that represents the protein's secondary amines wavenumber. Also, VAN (0.1%) decreased significantly A. platensis α-helix to 16.60 ± 0.52% compared to the control with 19.83 ± 0.32%. While, 0.1% TC increased (P < 0.05) the viscosity to 2.52 ± 0.61 Pa.s. This work demonstrated that terpenes could differently affect the physicochemical structure of microalgae biomass. The RMS's uniqueness comes from its ability to evaluate the functionality of terpenes during microalgae cultivation. Besides, chemometrics led to focus on the most important variances.
本研究旨在评估生物活性萜类化合物对螺旋藻（钝顶节旋藻；AP）和小球藻（普通小球藻；CV）生物量的功能影响。用0.01%、0.05%和0.1%的百里香酚（THY）、反式肉桂醛（TC）、薄荷醇（MEN）和香草醛（VAN）处理这两种微藻。拉曼显微光谱（RMS）与其他物理化学方法相关联，以确认其作用机制。结果显示，0.1%的THY使代表蛋白质仲胺波数的1196 cm处的RMS强度降低（P < 0.05）。此外，与对照的19.83±0.32%相比，0.1%的VAN使钝顶节旋藻的α-螺旋显著降低至16.60±0.52%。而0.1%的TC使粘度增加（P < 0.05）至2.52±0.61 Pa·s。这项工作表明，萜类化合物可对微藻生物量的物理化学结构产生不同影响。RMS的独特之处在于其能够评估微藻培养过程中萜类化合物的功能。此外，化学计量学有助于聚焦最重要的差异。
