Zhang Ying, Jia Xiuzhen, Chen Ximin, Liu Yajuan, Zhao Zifu, Hao Jingyu, Wu Rui, Feng Haotian, Ren Xiangnan
Department of Nutrition and Food Hygiene, School of Public Health, Jilin University, Changchun, China.
Yili Innovation Center, Inner Mongolia Yili Industrial Group Co., Ltd., Hohhot, China.
Ann Palliat Med. 2021 Apr;10(4):4572-4581. doi: 10.21037/apm-21-663.
L-theanine (L-THE), a natural amino acid found in green tea, has been shown to improve anxiety and sleep. Neumentix proprietary spearmint extract (PSE), which is commonly found in beverage flavoring a pharmaceutical, also has a wide range of health benefits, including cognitive performance improvement.
Four experiments tested the effects of L-THE and PSE on sleep: a direct sleeping test, pentobarbital-induced sleeping test, sub-hypnotic pentobarbital-induced sleeping test, and sodium barbital-induced sleeping test. Presence of neurotransmitters in brain tissue was detected by liquid chromatography mass spectroscopy (HP LC-MS) during these studies.
Pentobarbital-induced sleeping and sodium barbital-induced sleeping tests examined the potential effect of L-THE/PSE mixture on synergistic sleep, while neurotransmitter levels in the brain were determined by the high performance liquid chromatography/mass spectroscopy (HPLC/MS) method. L-THE and L-THE/PSE mixture showed increased sleep duration and shortened sleep latency when co-administrated with pentobarbital or sodium barbital. The mixture also increased sleeping rate when co-administrated with the pentobarbital at sub-hypnotic dose. Additionally, the L-THE, PSE and L-THE/PSE mixture significantly increased the concentrations of acetylcholine (Ach), γ-aminobutyric acid (GABA), and decreased the concentration of serotonin (5-HT) in the brain.
These data demonstrated that L-THE/PSE mixture regulates sleep disorders via the GABA receptor and neurotransmitter systems.
L-茶氨酸（L-THE）是绿茶中发现的一种天然氨基酸，已被证明可改善焦虑和睡眠。Neumentix专有留兰香提取物（PSE）常见于饮料调味剂和药物中，也具有广泛的健康益处，包括改善认知表现。
四项实验测试了L-THE和PSE对睡眠的影响：直接睡眠测试、戊巴比妥诱导睡眠测试、亚催眠剂量戊巴比妥诱导睡眠测试和巴比妥钠诱导睡眠测试。在这些研究中，通过液相色谱质谱法（HP LC-MS）检测脑组织中神经递质的存在。
戊巴比妥诱导睡眠测试和巴比妥钠诱导睡眠测试研究了L-THE/PSE混合物对协同睡眠的潜在影响，而大脑中的神经递质水平则通过高效液相色谱/质谱法（HPLC/MS）测定。与戊巴比妥或巴比妥钠共同给药时，L-THE和L-THE/PSE混合物显示出睡眠时间延长和睡眠潜伏期缩短。该混合物与亚催眠剂量的戊巴比妥共同给药时，睡眠率也有所提高。此外，L-THE、PSE和L-THE/PSE混合物显著增加了大脑中乙酰胆碱（Ach）、γ-氨基丁酸（GABA）的浓度，并降低了血清素（5-HT）的浓度。
这些数据表明，L-THE/PSE混合物通过GABA受体和神经递质系统调节睡眠障碍。
