Department of plastic and reconstructive surgery, Shanghai Ninth People's Hospital, Shanghai JiaoTong University school of medicine, 639, Zhizaoju road, 200011 Shanghai, China.
Department of plastic and reconstructive surgery, Shanghai Ninth People's Hospital, Shanghai JiaoTong University school of medicine, 639, Zhizaoju road, 200011 Shanghai, China.
Ann Chir Plast Esthet. 2021 Dec;66(6):440-446. doi: 10.1016/j.anplas.2021.03.007. Epub 2021 May 7.
Improving the survival rate of fat grafts is yet a difficult problem in the field of autologous fat transplantation. Prevailing methods such as making nanofat and SVF are time-consuming. Hence, the role of additives application in the improvement of fat graft survival during fat transplantation was considered and preliminarily evaluated in a rabbit animal model.
A rabbit animal model was established where rabbit ears were injected with a mixture of 1.5mL of adipose tissue and 1mL of saline (group A), 1.5mL of adipose tissue and 1mL of botulinum toxin A (BoNTA) (group B), 1.5mL of adipose tissue and 1mL of prostaglandin E2 (groupC), 1.5mL of adipose tissue and 1mL of PDRN (group D) respectively. Then, the extents of neovascularization and inflammation were evaluated on the 7th, 14th, 28th, 42nd, 56th and 70th day after injection by ELISA assays and H&E and immunofluorescence staining.
The results showed that pre-treatment with BoNTA, prostaglandin E2 and PDRN improved graft volume and weight. The H&E and immunofluorescence staining revealed that BoNTA, prostaglandin E2 and PDRN improved the graft angiogenesis. Simultaneously, TNF-α expression level detected by ELISA was the lowest in the PDRN group.
Henceforth, the present preliminary study suggests that pre-transplantation treatment with BoNTA, prostaglandin E2 and PDRN can improve the fat graft angiogenesis and graft integrity, whereby the effect of adding PDRN may be significant.
提高脂肪移植物的存活率仍然是自体脂肪移植领域的一个难题。目前流行的方法如制作纳米脂肪和 SVF 比较耗时。因此，在兔动物模型中，考虑并初步评估了添加剂应用在改善脂肪移植过程中脂肪移植物存活率中的作用。
建立兔动物模型，兔耳内分别注射 1.5mL 脂肪组织和 1mL 生理盐水（A 组）、1.5mL 脂肪组织和 1mL 肉毒毒素 A（BoNTA，B 组）、1.5mL 脂肪组织和 1mL 前列腺素 E2（C 组）、1.5mL 脂肪组织和 1mL PDRN（D 组）。然后，通过 ELISA 检测和 H&E 及免疫荧光染色，在注射后第 7、14、28、42、56 和 70 天评估新生血管形成和炎症的程度。
结果表明，BoNTA、前列腺素 E2 和 PDRN 预处理可提高移植物体积和重量。H&E 和免疫荧光染色显示，BoNTA、前列腺素 E2 和 PDRN 可促进移植物血管生成。同时，ELISA 检测到的 TNF-α 表达水平在 PDRN 组最低。
因此，本初步研究表明，BoNTA、前列腺素 E2 和 PDRN 预处理可改善脂肪移植物的血管生成和移植物完整性，其中添加 PDRN 的效果可能更为显著。
