Sivakumar Muniapillai, Yoithapprabhunath Thuckanaickenpalayam Ragunathan, Nirmal Ramadas Madhavan, Veeravarmal Veeran, Dineshshankar Janardhanam, Amsaveni Ramamoorthy
Department of Oral and Maxillofacial Pathology, Madha Dental College and Hospital, Chennai, Tamil Nadu, India.
Department of Oral and Maxillofacial Pathology, Vivekanandha Dental College for Women, Namakkal, Tamil Nadu, India.
J Oral Maxillofac Pathol. 2020 Sep-Dec;24(3):466-471. doi: 10.4103/jomfp.JOMFP_150_20. Epub 2021 Jan 9.
Ameloblastoma among benign tumors holds a unique position by its locally destructive and invasive nature. Tumors that originate from the odontogenic apparatus or its remnants in the jaws show diverse clinical presentations, behavior and histologic patterns. The differed biological behavior behind follicular and plexiform ameloblastomas has never attained completeness because of the lack of rhythmic correlation regarding the exact mechanism. Nuclear factor-kappa B (NF-κB) pathways play a crucial role in survival, death and differentiation during physiologic and pathologic conditions. With this background, the study has been aimed to investigate the expression of NF-κB in follicular and plexiform ameloblastomas.
The objective of this study was to analyze the immunohistochemical expression pattern of NF-κB in ameloblastoma and to compare the immunohistochemical expression pattern of NF-κB among the histological types of ameloblastoma, follicular and plexiform patterns.
Total 20 ameloblastomas (10 follicular, 10 plexiform) were immunostained with antihuman NF-κB p65 mouse IgG monoclonal antibody, and the pattern of staining is statistically analyzed using Chi-square test with the level of significance ( < 0.05).
Twelve (3 follicular, 9 plexiform) out of 20 ameloblastomas showed immunoreactivity to NF-κB p65. In ameloblastoma, only the peripheral preameloblast-like tall columnar cells showed reactivity, whereas the stellate reticulum-like cells are immunonegative. The staining pattern was membranous in the immunoreactive cells. The results were studied with the associated and inducing pathways from the literature, and a possible mechanism has been proposed.
The expression pattern of NF-κB was found to be higher in plexiform ameloblastoma than follicular ameloblastoma.
成釉细胞瘤在良性肿瘤中因其局部破坏性和侵袭性而占据独特地位。起源于颌骨牙源性器官或其残余组织的肿瘤表现出多样的临床表现、行为和组织学模式。由于缺乏关于确切机制的节律相关性，滤泡型和丛状型成釉细胞瘤背后不同的生物学行为尚未完全明确。核因子-κB（NF-κB）通路在生理和病理条件下的存活、死亡和分化过程中起关键作用。在此背景下，本研究旨在探讨NF-κB在滤泡型和丛状型成釉细胞瘤中的表达。
本研究的目的是分析NF-κB在成釉细胞瘤中的免疫组化表达模式，并比较NF-κB在成釉细胞瘤的组织学类型（滤泡型和丛状型）中的免疫组化表达模式。
用抗人NF-κB p65小鼠IgG单克隆抗体对20例成釉细胞瘤（10例滤泡型，10例丛状型）进行免疫染色，采用卡方检验对染色模式进行统计学分析，显著性水平为<0.05。
20例成釉细胞瘤中有12例（3例滤泡型，9例丛状型）对NF-κB p65呈免疫反应性。在成釉细胞瘤中，仅周边的前成釉细胞样高柱状细胞呈反应性，而星网状细胞呈免疫阴性。免疫反应性细胞中的染色模式为膜性。结合文献中的相关和诱导途径对结果进行了研究，并提出了一种可能的机制。
发现丛状型成釉细胞瘤中NF-κB的表达模式高于滤泡型成釉细胞瘤。
