Chaleshi Vahid, Asadzadeh Aghdaei Hamid, Nourian Mahyar, Iravani Shahrokh, Jalaeikhoo Hasan, Rajaeinejad Mohsen, Khoshdel Ali Reza, Naghoosi Hamed
Research Center for Cancer Screening and Epidemiology, AJA University of Medical Sciences, Tehran, Iran.
Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Gastroenterol Hepatol Bed Bench. 2021 Spring;14(2):108-114.
The aim of this study was to evaluate the expression of and the relationship between its expression with clinical characteristics in an Iranian gastric cancer patient.
Long non-coding RNAs (LncRNAs) play critical roles in the initiation and development of gastric cancer. Metastasis-associated lung adenocarcinoma transcript 1 () is a highly conserved lncRNA and plays key roles in various types of human cancer. However, our understanding of the role of lncRNAs in the occurrence and development of gastric cancer is not fully clear.
This cross-sectional study was performed on 41 gastric tumor tissue samples with matched normal adjacent tumor tissues. The RNA level of lncRNA gene was assessed using quantitative Real-time polymerase chain reaction. was used as an internal control. The 2 method was adopted to determine expression fold changes.
A significant association was observed between the levels of in gastric tumor tissues compared with normal adjacent tissues (mean= 1.558, = 0.014). In addition, clinicopathologic data on RNA expression levels in gastric cancer tissues was evaluated. No significant association was observed between the relative expression of and the stage, grade, infection, and tumor size groups among gastric cancer patients (= 0.82, = 0.904, = 0.407, and = 0.701, respectively).
The current results showed that has a significant association in gastric cancer. The expression of may be used as a diagnostic biomarker for monitoring gastric cancer patients.
本研究旨在评估一名伊朗胃癌患者中[具体长链非编码RNA名称]的表达情况及其表达与临床特征之间的关系。
长链非编码RNA（LncRNAs）在胃癌的发生和发展中起关键作用。转移相关肺腺癌转录本1（[具体长链非编码RNA名称]）是一种高度保守的lncRNA，在多种类型的人类癌症中起关键作用。然而，我们对lncRNAs在胃癌发生和发展中的作用的理解尚不完全清楚。
本横断面研究对41例胃肿瘤组织样本及其匹配的正常相邻肿瘤组织进行。使用定量实时聚合酶链反应评估lncRNA[具体长链非编码RNA名称]基因的RNA水平。[具体内参名称]用作内对照。采用2-△△CT法确定表达倍数变化。
与正常相邻组织相比，胃肿瘤组织中[具体长链非编码RNA名称]的水平存在显著相关性（平均值 = 1.558，P = 0.014）。此外，评估了胃癌组织中[具体长链非编码RNA名称]RNA表达水平的临床病理数据。在胃癌患者中，[具体长链非编码RNA名称]的相对表达与分期、分级、[具体感染名称]感染和肿瘤大小组之间未观察到显著相关性（P分别为0.82、0.904、0.407和0.701）。
目前的结果表明[具体长链非编码RNA名称]在胃癌中具有显著相关性。[具体长链非编码RNA名称]的表达可作为监测胃癌患者的诊断生物标志物。
