Department of Biochemistry and Microbiology, University of Fort Hare, Alice, Eastern Cape, 5700, South Africa.
Medicinal Plant and Economic Development (MPED) Research Centre, University of Fort Hare, Alice, Eastern Cape, 5700, South Africa.
F1000Res. 2020 Oct 15;9:1240. doi: 10.12688/f1000research.26855.2. eCollection 2020.
Hilliard & B.L. Burtt has been listed in a survey of plants used in traditional medicine for the treatment of type 2 diabetes in the Eastern Cape of South Africa. In this study, the antidiabetic potentials of ethanol, cold aqueous (CAQ) and boiled aqueous (BAQ) extracts of were investigated. The cytotoxic and glucose utilization effects of the extracts were evaluated using L6 myocytes and HepG2 (C3A) hepatocytes. α-amylase, α-glucosidase and lipase inhibition assays were also carried out. The ethanol extract showed significant cytotoxic effects in the treated cells. Both BAQ and CAQ extracts significantly increased glucose uptake in L6 and C3A cell lines. The CAQ extract enhanced glucose uptake more in the L6 myocytes than in the C3A cell-lines hepatocytes. The BAQ extract showed higher levels of inhibition on α-amylase and α-glucosidase than CAQ. The activities were not significantly different from acarbose. However, BAQ showed lower lipase inhibition than acarbose (p<0.05). The BAQ and CAQ extracts of may, therefore, contain pharmacologically active and relatively non-toxic hypoglycaemic chemicals, which may be effective substitutes in the treatment of diabetes mellitus.
在南非东开普省的一项针对用于治疗 2 型糖尿病的传统医学植物的调查中，希利亚德和 B.L.伯蒂被列入其中。在这项研究中，研究了乙醇、冷水性（CAQ）和煮沸水性（BAQ）提取物的抗糖尿病潜力。使用 L6 肌细胞和 HepG2（C3A）肝细胞评估了提取物的细胞毒性和葡萄糖利用作用。还进行了α-淀粉酶、α-葡萄糖苷酶和脂肪酶抑制试验。乙醇提取物在处理细胞中表现出显著的细胞毒性作用。BAQ 和 CAQ 提取物均能显著增加 L6 和 C3A 细胞系中的葡萄糖摄取量。CAQ 提取物在 L6 肌细胞中的葡萄糖摄取量增加高于 C3A 细胞系中的肝细胞。BAQ 提取物对α-淀粉酶和α-葡萄糖苷酶的抑制作用高于 CAQ。其活性与阿卡波糖无显著差异。然而，BAQ 的脂肪酶抑制作用低于阿卡波糖（p<0.05）。因此，可能含有药理学上有效且相对无毒的降血糖化学物质，可作为糖尿病治疗的有效替代品。
