Adel Amera, El-Baz Ashraf, Shetaia Yousseria, Sorour Noha Mohamed
Food Safety Organization, Cairo, Egypt.
Département of Industrial Biotechnology, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Sadat City, 22857/79 Egypt.
3 Biotech. 2021 Nov;11(11):461. doi: 10.1007/s13205-021-03010-4. Epub 2021 Oct 16.
The widespread awareness of polyunsaturated fatty acids (PUFAs) benefits for human health has increased the need for their commercial production. Two oleaginous yeast were isolated from the Mediterranean Sea fish and Red Sea fish and , respectively. These marine candidates were identified by MALDI-TOF/MS biotyper as and . The effect of incubation temperature (7, 15, and 26 °C) and glucose concentration (3% and 8%) on their lipids content were investigated using Sulfo-Phospho-Vanillin (SPV) assay. Their intercellular lipids were visualized by fluorescence microscope using Nile-Red dye. and produced 20.04% and 26.79% of Linoleic acid, respectively, on normal Basal-Defatted Medium (BDM). Linoleic acid (21.4-22.7%) and α-Linolenic acid (7.5-10.8%) were produced by and . on normal BDM at 15 °C. High-Glucose BDM induced a positive effect on the total lipids production that reached its maximum of 48% and 54% by and . , respectively, grown at 15 °C. Remarkably, 12.12% of long-chain 15-Docosenoic acid (C) and 21.49% of Tricosanoic acid (C) were detected in the FAs profile of , when grown on normal BDM at 26 °C. The present study is the first one reporting the FAs profile of the Egyptian Marine , and its capability to accumulate high amounts of lipids under appropriate fermentation conditions; thus, it could be considered for scaling up production.
The online version contains supplementary material available at 10.1007/s13205-021-03010-4.
多不饱和脂肪酸（PUFAs）对人类健康有益这一广泛认知增加了其商业生产的需求。分别从地中海鱼类和红海鱼类中分离出两种产油酵母。通过基质辅助激光解吸电离飞行时间质谱（MALDI-TOF/MS）生物分型法将这些海洋候选菌株鉴定为[具体菌株名称1]和[具体菌株名称2]。使用磺基磷香草醛（SPV）测定法研究了培养温度（7、15和26℃）和葡萄糖浓度（3%和8%）对其脂质含量的影响。使用尼罗红染料通过荧光显微镜观察它们的细胞内脂质。在正常基础脱脂培养基（BDM）上，[菌株名称1]和[菌株名称2]分别产生了20.04%和26.79%的亚油酸。在15℃的正常BDM上，[菌株名称3]产生了21.4 - 22.7%的亚油酸和7.5-10.8%的α-亚麻酸。高糖BDM对总脂质生产有积极影响，在15℃下生长的[菌株名称1]和[菌株名称2]分别使总脂质产量达到最大值48%和54%。值得注意的是，当在26℃的正常BDM上生长时，在[菌株名称4]的脂肪酸谱中检测到12.12%的长链15-二十二碳烯酸（C22:1）和21.49%的二十三烷酸（C23:0）。本研究是首次报道埃及海洋[菌株名称4]的脂肪酸谱及其在适当发酵条件下积累大量脂质的能力；因此，可考虑扩大生产规模。
在线版本包含可在10.1007/s13205-021-03010-4获取的补充材料。
