School of Resources and Environmental Engineering, Anhui University, Hefei, Anhui Province, 230601, China; Key Laboratory of Wetland Ecological Protection and Restoration, China; Anhui Province Engineering Laboratory for Mine Ecological Remediation, China.
School of Resources and Environmental Engineering, Anhui University, Hefei, Anhui Province, 230601, China; Key Laboratory of Wetland Ecological Protection and Restoration, China; Anhui Province Engineering Laboratory for Mine Ecological Remediation, China.
J Environ Manage. 2020 Feb 1;255:109933. doi: 10.1016/j.jenvman.2019.109933. Epub 2019 Dec 3.
The surface of leaf, also known as phyllosphere, harbors diverse microbial communities which include both beneficial microorganisms promoting plants growth and harmful microorganisms, such as plant pathogens and human pathogens. Several studies have investigated the interaction between plants and human pathogens, while few works have focused on the quantitative analysis of pathogenic bacteria. On the basis of real-time polymerase chain reaction (qPCR), this study aimed to evaluate the abundance of following genes: the nuc and pvl of Staphylococcus aureus, the lytA and psaA of Streptococcus pneumoniae, and the ttr and invA of Salmonella enterica in the phyllosphere of four landscape plants (Nandina domestica, Rhododendron pulchrum, Photinia serrulata, and Cinnamomum camphora) growing in two habitats. Our results indicated that the relative abundance of pathogenic genes in the phyllosphere ranged from 10 to 10. The specific genes of S. aureus, S. pneumoniae and S. enterica in landscape plants were pvl, lytA and ttr, respectively. The two pathogenic genes of S. pneumoniae and the 16S rRNA gene were mainly affected by habitats, host species, and habitats-species interaction. Moreover, for the abundance of lytA and 16S rRNA, results showed that plants present in roadside with traffic pollution were relatively higher than that of campus with less pollution. The N. domestica and C. camphora were recommended for planting along the roadsides due to lower abundance of pathogenic genes. However, we have observed no significant difference in the abundance of pathogenic genes among four plants in the campus. Thereby, this study provided a valuable reference for selecting landscape plants in view of human health.
叶片表面，也称为叶际，栖息着多样化的微生物群落，包括促进植物生长的有益微生物和有害微生物，如植物病原体和人类病原体。已有多项研究调查了植物与人类病原体之间的相互作用，而很少有研究关注致病菌的定量分析。本研究基于实时聚合酶链反应（qPCR），旨在评估以下基因在四种景观植物（南天竹、锦绣杜鹃、红叶石楠和香樟）叶际中的丰度：金黄色葡萄球菌的 nuc 和 pvl 基因、肺炎链球菌的 lytA 和 psaA 基因、以及肠炎沙门氏菌的 ttr 和 invA 基因，这些植物生长在两种生境中。我们的结果表明，叶际中致病菌基因的相对丰度范围为 10 到 10。景观植物中金黄色葡萄球菌、肺炎链球菌和肠炎沙门氏菌的特定基因分别为 pvl、lytA 和 ttr。肺炎链球菌的两个致病基因和 16S rRNA 基因主要受生境、宿主物种和生境-物种相互作用的影响。此外，对于 lytA 和 16S rRNA 的丰度，结果表明，受到交通污染的路边植物相对高于污染较少的校园植物。由于致病基因丰度较低，建议在路边种植南天竹和香樟。然而，我们没有观察到校园中四种植物的致病基因丰度存在显著差异。因此，本研究为从人类健康角度选择景观植物提供了有价值的参考。
