Behl Rahul, Niranjan S K, Behl Jyotsna, Arora Reena, Singh P K, Vijh R K
ICAR-National Bureau of Animal Genetic Resources, Karnal, India.
Anim Biotechnol. 2022 Dec;33(7):1588-1590. doi: 10.1080/10495398.2021.1919131. Epub 2021 May 9.
The grey type donkeys of region of Uttar Pradesh in India were genetically characterized using ten heterologous microsatellite loci. At these loci the PCR product size ranged from 75-95 bp at locus HTG6 to 251-277 bp at locus COR18. The observed number of alleles varied from 4 (HTG15) to 10 (HTG7 and AHT4) with a mean of 7.50 ± 1.96. The observed heterozygosity ranged from 0.46 (HTG6 and NVHEQ54) to 0.79 (COR18) with a mean of 0.66 ± 0.12. The mean genetic diversity estimate (FIS) was 0.157. When these donkeys were compared on the basis of allelic frequency data at these loci to the brown type donkeys of Ladakh, Spiti and Rayalseema regions in the union territories/states of Ladakh, Himachal Pradesh and Andhra Pradesh, respectively, they clustered independently from these three donkey populations in a dendrogram based on Goldstein's average square distances indicating their genetic distinctness.
利用10个异源微卫星位点对印度北方邦地区的灰色品种驴进行了遗传特征分析。在这些位点上，PCR产物大小在HTG6位点为75 - 95 bp，在COR18位点为251 - 277 bp。观察到的等位基因数量从4个（HTG15）到10个（HTG7和AHT4）不等，平均值为7.50±1.96。观察到的杂合度范围从0.46（HTG6和NVHEQ54）到0.79（COR18），平均值为0.66±0.12。平均遗传多样性估计值（FIS）为0.157。当根据这些位点的等位基因频率数据，将这些驴与分别来自拉达克、喜马偕尔邦和安得拉邦联合属地/邦的拉达克、斯皮提和拉亚尔西马地区的棕色品种驴进行比较时，在基于戈尔茨坦平均平方距离的聚类图中，它们与这三个驴种群独立聚类，表明它们在遗传上具有独特性。
