Animal Genetics Division, ICAR-National Bureau of Animal Genetic Resource, Karnal, Haryana, 132001, India.
Animal Genetic Resources Division, ICAR-National Bureau of Animal Genetic Resource, Karnal, Haryana, 132001, India.
Sci Rep. 2024 May 11;14(1):10794. doi: 10.1038/s41598-024-61542-0.
The primary objective of Sustainable Development Goal target 2.5 established by the United Nations is to ensure the preservation of genetic diversity in domesticated animals. The ICAR-National Bureau of Animal Genetic Resources in India has been actively engaged in the conservation of cattle and buffalo bull semen for long-term storage. This present study aimed to assess the genetic diversity present in the conserved cattle bull semen, which would aid in determining the most suitable strategy for future conservation management. A total of 192 bull semen belonging to 19 cattle breeds were selected to evaluate genetic diversity using 17 pairs of FAO recommended microsatellite primers. Total 267 alleles were detected across all the samples which indicates substantial amount of allelic variation is being maintained in conserved bulls. Further, all cattle bulls semen conserved showed higher observed heterozygosity than expected heterozygosity which indicates excess genetic diversity in all the populations. The F, F and F value across the loci and population is 0.146 ± 0.009, 0.054 ± 0.038, and - 0.105 ± 0.035, respectively, which suggests lack of inbreeding in conserved cattle bull semen. This study has established genetic diversity in conserved cattle semen samples to achieve sustainable development goals. In addition, it provides compelling evidence that the current approach for conserving cattle bull semen is heading in the correct direction.
联合国制定的可持续发展目标 2.5 的主要目标是确保家养动物遗传多样性得到保护。印度农业研究理事会-国家动物遗传资源局长期以来一直致力于牛和水牛公牛精液的保存，以便进行长期储存。本研究旨在评估保存的公牛精液中的遗传多样性，这将有助于确定未来保护管理的最合适策略。选择了 192 份来自 19 个牛品种的公牛精液样本，使用 17 对粮农组织推荐的微卫星引物评估遗传多样性。在所有样本中共检测到 267 个等位基因，这表明在保存的公牛中保持着大量的等位基因变异。此外，所有保存的公牛精液都表现出高于预期的杂合度，这表明所有群体都存在过多的遗传多样性。在所有位点和群体中，F、F 和 F 值分别为 0.146±0.009、0.054±0.038 和-0.105±0.035，这表明保存的公牛精液中不存在近交。本研究在保存的牛精液样本中建立了遗传多样性，以实现可持续发展目标。此外，它提供了有力的证据表明，目前保存牛公牛精液的方法是正确的。
