Zhang R F, Xie W M, Zhang T, Lei C Z
National Experiment Teaching Center of Biology, College of Life Science, Hubei Normal University, Huangshi, Hubei, China.
Department of Basic Medicine, Pingliang Medical College, Pingliang, Gansu, China.
Genet Mol Res. 2016 Jun 24;15(2):gmr8291. doi: 10.4238/gmr.15028291.
To reveal the genetic diversity and phylogenetic relationships between Chinese donkey breeds, 415 individuals representing ten breeds were investigated using ten microsatellite markers. The observed number of alleles, mean effective number of alleles (NE), mean expected heterozygosity (HE), and polymorphic information content (PIC) of each breed and polymorphic locus were analyzed. The results showed that seven (HTG7, HTG10, AHT4, HTG6, HMS6, HMS3, and HMS7) of ten microsatellite loci were polymorphic. The mean PIC, HE, and NE of seven polymorphic loci for the ten donkey breeds were 0.7679, 0.8072, and 6.0275, respectively. These results suggest that domestic Chinese donkey breeds possess higher levels of genetic diversity and heterozygosity than foreign donkeys. A neighbor-joining tree based on Nei's standard genetic distance showed that there was close genetic distance among Xinjiang, Qingyang, Xiji, and Guanzhong donkey breeds. In addition, Mongolia and Dezhou donkey breeds were placed in the same category. The phylogenetic tree revealed that the genetic relationships between Chinese donkey breeds are consistent with their geographic distribution and breeding history.
为揭示中国驴品种之间的遗传多样性和系统发育关系，利用10个微卫星标记对代表10个品种的415头个体进行了研究。分析了每个品种和多态性位点的观察等位基因数、平均有效等位基因数（NE）、平均期望杂合度（HE）和多态信息含量（PIC）。结果表明，10个微卫星位点中有7个（HTG7、HTG10、AHT4、HTG6、HMS6、HMS3和HMS7）具有多态性。10个驴品种的7个多态性位点的平均PIC、HE和NE分别为0.7679、0.8072和6.0275。这些结果表明，中国家驴品种比国外驴具有更高水平的遗传多样性和杂合度。基于Nei标准遗传距离构建的邻接树表明，新疆驴、庆阳驴、西吉驴和关中驴品种之间的遗传距离较近。此外，蒙古驴和德州驴品种归为同一类。系统发育树显示，中国驴品种之间的遗传关系与其地理分布和育种历史一致。
