National Institute of Alcohol Abuse and Alcoholism, Section of Sensory Science and Metabolism.
Monell Chemical Senses Center.
J Vis Exp. 2021 Apr 21(170). doi: 10.3791/62384.
This paper describes a two-alternative, forced-choice, staircase, tracking procedure, called the Taste Detection Threshold (TDT) test, that provides a reliable measure of sweet, salty, and umami taste detection thresholds from childhood to adulthood. Advantages of the method include procedures that are identical for children and adults, thus allowing the determination of age-related and individual differences in taste perception, if any, and tasks that can be completed in a relatively short time frame, do not rely on continuous attention or require memorization, control for subjective response biases, and minimize the impact of language development. After a 1 hour fast, participants are presented with pairs of solutions; in each pair, one solution is water, and the other solution contains varying concentrations of the tastant. Using a whole-mouth tasting method, participants taste each solution (without swallowing and with rinsing between tastings) and then point to the solution with a taste or that tastes different from water. The concentration of the stimulus in the subsequent pair increases after a single incorrect response and decreases after two consecutive correct responses. A reversal occurs when the concentration sequence changes direction. The task is deemed completed after the occurrence of four reversals, provided there are a maximum of two dilution steps between two successive reversals, and the series of reversals do not form an ascending pattern. These additional criteria ensure greater reliability in outcomes. The TDT is then calculated as the geometric mean of the concentrations of the four reversals. This method has real-world relevance as it provides information on a dimension of taste perception that is independent of hedonics, and that can change with aging and certain disease states, making it a valuable psychophysical test.
本文描述了一种二选一、强制选择、阶梯式、追踪程序,称为味觉检测阈值(TDT)测试,它提供了一种可靠的方法,可以测量从儿童到成年的甜味、咸味和鲜味的味觉检测阈值。该方法的优点包括儿童和成人的程序相同,因此可以确定味觉感知的年龄相关和个体差异(如果有的话),以及可以在相对较短的时间内完成的任务,不需要连续注意或记忆,控制主观反应偏差,并最大限度地减少语言发展的影响。在禁食 1 小时后,参与者会呈现成对的溶液;在每对中,一种溶液是水,另一种溶液含有不同浓度的味觉剂。参与者采用全口品尝的方法品尝每种溶液(不吞咽,品尝之间漱口),然后指向有味道或与水味道不同的溶液。在单次错误响应后,下一对刺激物的浓度会增加,在两次连续正确响应后会降低。如果浓度序列改变方向,则会发生反转。只有在发生四次反转且两次连续反转之间最多有两个稀释步骤,并且反转序列没有形成上升模式的情况下,任务才被视为完成。这些额外的标准确保了结果的更高可靠性。然后将 TDT 计算为四个反转的浓度的几何平均值。该方法具有实际意义,因为它提供了与享乐主义无关的味觉感知维度的信息,并且可以随着年龄的增长和某些疾病状态而改变,因此是一种有价值的心理物理测试。