Lindner C, Lichtenberg V, Westhof G, Braendle W, Bettendorf G
Abteilung für klinische und experimentelle Endokrinologie, Universitäts-Frauenklinik Hamburg, Germany.
Horm Metab Res. 1988 Apr;20(4):243-6. doi: 10.1055/s-2007-1010803.
The induction of multiple follicular growth during ovarian stimulation for in vitro fertilization (IVF) implies follicular asynchrony. As a consequence oocytes of different quality are obtained. The maturity and fertilizability of oocytes cannot sufficiently be predicted by their morphological appearance under the light microscope. Looking for additional parameters of oocyte quality, FSH, hCG, estradiol (E2), progesterone (P), testosterone (T), prolactin (PRL) and cAMP were analysed in human follicular fluid (FF) containing a morphologically mature oocyte. The evaluation of the relationship between FF values and oocyte fertilization showed the following results: no differences of FSH, hCG, E2, P and T concentrations in FF between the group of fertilized and not fertilized ova. However, significant differences were detected for PRL and cAMP. In FF of fertilized oocytes PRL content was higher (38.8 +/- 2.2 vs 29.7 +/- 2.3 ng/ml, P less than 0.01) and cAMP level was lower (32.7 +/- 1.9 vs 59.8 +/- 7.4 pmol/ml, P less than 0.01) as compared with FF of unfertilizable oocytes. In conclusion PRL- and cAMP concentration of FF might be additional parameters of oocyte maturation and fertilizability.
在体外受精（IVF）的卵巢刺激过程中诱导多个卵泡生长意味着卵泡不同步。因此会获得不同质量的卵母细胞。卵母细胞的成熟度和受精能力无法通过其在光学显微镜下的形态外观充分预测。为了寻找卵母细胞质量的其他参数，对含有形态学上成熟卵母细胞的人卵泡液（FF）中的促卵泡激素（FSH）、人绒毛膜促性腺激素（hCG）、雌二醇（E2）、孕酮（P）、睾酮（T）、催乳素（PRL）和环磷酸腺苷（cAMP）进行了分析。卵泡液值与卵母细胞受精之间关系的评估结果如下：受精和未受精卵母细胞组之间卵泡液中FSH、hCG、E2、P和T浓度无差异。然而，检测到PRL和cAMP有显著差异。与未受精的卵母细胞的卵泡液相比，受精的卵母细胞的卵泡液中PRL含量更高（38.8±2.2对29.7±2.3 ng/ml，P＜0.01），而cAMP水平更低（32.7±1.9对59.8±7.4 pmol/ml，P＜0.01）。总之，卵泡液中PRL和cAMP浓度可能是卵母细胞成熟度和受精能力的其他参数。
