Pharmaceutical Analysis Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
J Mol Recognit. 2021 Oct;34(10):e2903. doi: 10.1002/jmr.2903. Epub 2021 May 10.
A novel spectrofluorimetric sensing platform was designed for Ractopamine measurement in aqueous and plasma samples. d-penicillamine functionalized graphene quantum dots (DPA-GQDs) was utilized as a fluorescence probe, which was synthesized through the pyrolysis of citric acid in the presence of DPA. This one-pot down-top strategy causes to high-yield controllable synthesis method. The reaction time and probe concentration were optimized. Then, the fluorescence intensity of aqueous samples containing different Ractopamine concentrations and 500 ppm DPA-GQDs were measured at 25°C with an excitation wavelength of 274 nm. The sensing platform was also applied to detect Ractopamine in untreated plasma samples. The fluorescence spectroscopy technique responses indicated a linear relationship between the peak fluorescence intensity and ractopamine concentration in the range of 0.25-15 ppm with low limit of quantification of 0.25 ppm was for aqueous and plasma samples, respectively.
设计了一种新型的荧光传感平台，用于在水相和血浆样品中检测莱克多巴胺。二巯丁二酸功能化石墨烯量子点（DPA-GQDs）被用作荧光探针，通过柠檬酸在 DPA 存在下的热解合成。这种一锅法自上而下的策略导致了高产可控的合成方法。优化了反应时间和探针浓度。然后，在 25°C 下，用 274nm 的激发波长测量含有不同莱克多巴胺浓度和 500ppm DPA-GQDs 的水相样品的荧光强度。该传感平台还用于检测未经处理的血浆样品中的莱克多巴胺。荧光光谱技术响应表明，在 0.25-15ppm 的范围内，峰荧光强度与莱克多巴胺浓度之间呈线性关系，水相和血浆样品的定量下限分别为 0.25ppm。
