Albogami Bander
Biology Department, College of Sciences, Taif University, Taif 21944, Saudi Arabia.
Saudi J Biol Sci. 2023 Sep;30(9):103763. doi: 10.1016/j.sjbs.2023.103763. Epub 2023 Aug 5.
Trichinellosis is a serious food-borne parasitic disease with serious community health effects, mainly causing muscle damage with no recent approved treatment. This study aimed to assess the therapeutic effect of gallic acid (GA) as a potent antioxidant against the encysted phase of in male (BALB/c) mice alone or combined with albendazole (ALB) and to detect their synergistic effects on the histology and ultrastructure of skeletal and cardiac muscles and some biochemical blood analyses. Forty male mice were randomly divided into five groups (8 mice/group). 1 group: the negative control received only distilled water, 2 group: the positive control (infected control group without treatment), 3 group: infected group plus treatment with ALB (50 mg Kg orally), and 4 group: infected group and then treated with GA (30 mg Kg orally) and finally 5 infected group treated with a combination of both ALB and GA. Aspartate and Alanine aminotransferase, Lactate dehydrogenase, alkaline phosphatase, C-reactive protein, Interleukin-4 and Creatine kinase were used as biochemical markers of hepatic and cardiac toxicity and inflammation. Malondialdehyde level, catalase, superoxide dismutase, and glutathione peroxidase were evaluated in heart tissue homogenates beside histological and ultra-structural examination of heart and skeletal muscles beside parasitological analyses. Results showed that the reduction % of sp. larvae g in muscles of the group treated with the combination of GA and ALB showed overall reduction percentages. Oral administration of 30 mg kg of GA led to infection reduction of than ALB treated group. Both administration of ALB beside GA showed the best treatment group that resulted in high infection reduction besides amelioration of both biochemical markers and restoration of histological and ultrastructures to normal state. In conclusion, GA is highly effective against which could be a promising alternative antioxidant drug and the GA effect was higher in the case of combination with ALB. This experiment provides a basis for further exploration of potent activities of other antioxidants against different phases of and the reduction of any health hazards prospectively.
旋毛虫病是一种严重的食源性寄生虫病，对社区健康有严重影响，主要导致肌肉损伤，目前尚无近期获批的治疗方法。本研究旨在评估没食子酸（GA）作为一种强效抗氧化剂对雄性（BALB/c）小鼠旋毛虫包囊期的治疗效果，单独使用或与阿苯达唑（ALB）联合使用，并检测它们对骨骼肌和心肌的组织学和超微结构以及一些血液生化分析的协同作用。将40只雄性小鼠随机分为五组（每组8只）。第1组：阴性对照组仅接受蒸馏水；第2组：阳性对照组（未治疗的感染对照组）；第3组：感染组加ALB治疗（口服50 mg/kg）；第4组：感染组，然后用GA治疗（口服30 mg/kg）；最后第5组感染组用ALB和GA联合治疗。天冬氨酸转氨酶、丙氨酸转氨酶、乳酸脱氢酶、碱性磷酸酶、C反应蛋白、白细胞介素-4和肌酸激酶用作肝毒性、心脏毒性和炎症的生化标志物。除了对心脏和骨骼肌进行组织学和超微结构检查以及寄生虫学分析外，还评估了心脏组织匀浆中的丙二醛水平、过氧化氢酶、超氧化物歧化酶和谷胱甘肽过氧化物酶。结果表明，GA和ALB联合治疗组肌肉中旋毛虫幼虫的减少百分比显示出总体减少率。口服30 mg/kg的GA导致感染减少率高于ALB治疗组。GA联合ALB给药显示出最佳治疗组，除了改善生化标志物以及将组织学和超微结构恢复到正常状态外，还导致高感染减少率。总之，GA对旋毛虫具有高度有效性，这可能是一种有前途的替代抗氧化药物，并且GA与ALB联合使用时效果更高。本实验为进一步探索其他抗氧化剂对旋毛虫不同阶段的有效活性以及前瞻性地减少任何健康危害提供了依据。
