Department of Pharmacy, College of Health Sciences, Arsi University, Assela, Ethiopia.
Department of Pharmacology and Clinical Pharmacy, College of Health Sciences, Addis Ababa University, Addis Ababa, Ethiopia.
Ann Clin Microbiol Antimicrob. 2021 May 10;20(1):33. doi: 10.1186/s12941-021-00440-z.
Globally, surgical site infections are the most reported healthcare-associated infection and common surgical complication. In developing countries such as Ethiopia, there is a paucity of published reports on the microbiologic profile and resistance patterns of an isolates.
This study aimed at assessing the bacterial profile and antimicrobial susceptibility patterns of isolates among patients diagnosed with surgical site infection at Jimma Medical Center in Ethiopia.
A prospective cohort study was employed among adult patients who underwent either elective or emergency surgical procedures. All the eligible patients were followed for 30 days for the occurrence of surgical site infection (SSI). From those who developed SSI, infected wound specimens were collected and studied bacteriologically.
Of 251 study participants, 126 (50.2%) of them were females. The mean ± SD age of the patients was 38 ± 16.30 years. The overall postoperative surgical site infection rate was 21.1% and of these 71.7% (38/53) were culture positive. On gram stain analysis, 78% of them were Gram-negative, 11.5% were Gram-positive and 10.5% were a mixture of two microbial growths. Escherichia coli accounted for (21.43%), followed by Pseudomonas aeruginosa (19.05%), Proteus species (spp.) 14.29%), Staphylococcus aureus (11.90%), Klebsiella species (11.90%), Citrobacter spp. (9.5%), streptococcal spp. (7.14%), Coagulase-negative S. aureus (CoNS) (2.38%) CONCLUSION: Gram-negative bacteria were the most dominant isolates from surgical sites in the study area. Among the Gram-negative bacilli, Escherichia coli were the most common bacteria causing surgical site infection. As there is high antibiotic resistance observed in the current study, it is necessary for routine microbial analysis of samples and their antibiogram.
在全球范围内，手术部位感染是报告最多的与医疗保健相关的感染和常见的手术并发症。在埃塞俄比亚等发展中国家，关于分离物的微生物分布和耐药模式的已发表报告很少。
本研究旨在评估在埃塞俄比亚 Jimma 医疗中心诊断为手术部位感染的患者中分离物的细菌分布和抗菌药物敏感性模式。
采用前瞻性队列研究，纳入接受择期或急诊手术的成年患者。所有符合条件的患者在 30 天内进行随访，以确定是否发生手术部位感染（SSI）。对发生 SSI 的患者，采集感染性伤口标本进行细菌学研究。
在 251 名研究参与者中，有 126 名（50.2%）为女性。患者的平均年龄为 38 岁±16.30 岁。总的术后手术部位感染率为 21.1%，其中 71.7%（38/53）为培养阳性。革兰氏染色分析显示，其中 78%为革兰氏阴性菌，11.5%为革兰氏阳性菌，10.5%为两种微生物生长的混合物。大肠杆菌占（21.43%），其次是铜绿假单胞菌（19.05%）、变形杆菌属（14.29%）、金黄色葡萄球菌（11.90%）、克雷伯菌属（11.90%）、柠檬酸杆菌属（9.5%）、链球菌属（7.14%）、凝固酶阴性金黄色葡萄球菌（CoNS）（2.38%）。
在研究区域，革兰氏阴性菌是手术部位最主要的分离物。在革兰氏阴性杆菌中，大肠杆菌是引起手术部位感染最常见的细菌。由于本研究中观察到抗生素耐药性很高，因此有必要对样本进行常规微生物分析和药敏试验。
