Geng Ruiman, Xu Junlei, Jiang Jutang, Cheng Zhanchao, Sun Maosheng, Xia Nianhe, Gao Jian
Key Laboratory of National Forestry and Grassland Administration/Beijing for Bamboo & Rattan Science and Technology, International Center for Bamboo and Rattan, Beijing 100102, China.
Institute of Bamboo and Rattan, Southwest Forestry University, Kunming 650224, China.
Plants (Basel). 2024 Oct 17;13(20):2910. doi: 10.3390/plants13202910.
is a high-quality bamboo species that can be used for both bamboo shoots and wood. The nutritional components and flavors of vary from different geographical provenances. However, the unique biological characteristics of bamboo make morphological classification methods unsuitable for distinguishing them. Although the new cultivar 'Manxie No.1' has significant differences in the branch characteristics and the color of shoot sheaths compared to the , it still lacks precise genetic information at the molecular level. This study identified 231,789 microsatellite markers based on the whole genome of and analyzed their type composition and distribution on chromosomes in detail. Then, using TP-M13-SSR fluorescence-labeling technology, 34 pairs of polymorphic primers were screened to identify the new cultivar 'Manxie No.1' and 11 different geographical provenances of . We also constructed DNA fingerprinting profiles for them. At the same time, we mapped six polymorphic SSRs to the gene of , among which SSR673 was mapped to , which is related to plant immunity. The specific markers selected in this study can rapidly identify the provenances and the new cultivar of and help explore candidate genes related to some important traits.
是一种高品质的竹种，可用于竹笋和竹材。其营养成分和风味因地理来源不同而有所差异。然而，竹子独特的生物学特性使得形态分类方法不适用于区分它们。尽管新品种“满协1号”与[原文缺失相关品种名称]相比，在分枝特征和笋箨颜色上有显著差异，但在分子水平上仍缺乏精确的遗传信息。本研究基于[原文缺失相关竹种名称]的全基因组鉴定了231,789个微卫星标记，并详细分析了它们的类型组成和在染色体上的分布。然后，利用TP-M13-SSR荧光标记技术，筛选出34对多态性引物，用于鉴定新品种“满协1号”和[原文缺失相关竹种名称]的11个不同地理来源。我们还为它们构建了DNA指纹图谱。同时，我们将6个多态性SSR定位到[原文缺失相关竹种名称]的基因上，其中SSR673定位到与植物免疫相关的[原文缺失相关基因名称]上。本研究中筛选出的特异性标记可快速鉴定[原文缺失相关竹种名称]的地理来源和新品种，并有助于探索与一些重要性状相关的候选基因。
