Suppr超能文献

利用全基因组开发的简单序列重复标记鉴定(禾本科:竹亚科)新品种及不同种源

Identification of New Cultivar and Different Provenances of (Poaceae: Bambusoideae) Using Simple Sequence Repeats Developed from the Whole Genome.

作者信息

Geng Ruiman, Xu Junlei, Jiang Jutang, Cheng Zhanchao, Sun Maosheng, Xia Nianhe, Gao Jian

机构信息

Key Laboratory of National Forestry and Grassland Administration/Beijing for Bamboo & Rattan Science and Technology, International Center for Bamboo and Rattan, Beijing 100102, China.

Institute of Bamboo and Rattan, Southwest Forestry University, Kunming 650224, China.

出版信息

Plants (Basel). 2024 Oct 17;13(20):2910. doi: 10.3390/plants13202910.

Abstract

is a high-quality bamboo species that can be used for both bamboo shoots and wood. The nutritional components and flavors of vary from different geographical provenances. However, the unique biological characteristics of bamboo make morphological classification methods unsuitable for distinguishing them. Although the new cultivar 'Manxie No.1' has significant differences in the branch characteristics and the color of shoot sheaths compared to the , it still lacks precise genetic information at the molecular level. This study identified 231,789 microsatellite markers based on the whole genome of and analyzed their type composition and distribution on chromosomes in detail. Then, using TP-M13-SSR fluorescence-labeling technology, 34 pairs of polymorphic primers were screened to identify the new cultivar 'Manxie No.1' and 11 different geographical provenances of . We also constructed DNA fingerprinting profiles for them. At the same time, we mapped six polymorphic SSRs to the gene of , among which SSR673 was mapped to , which is related to plant immunity. The specific markers selected in this study can rapidly identify the provenances and the new cultivar of and help explore candidate genes related to some important traits.

摘要

是一种高品质的竹种,可用于竹笋和竹材。其营养成分和风味因地理来源不同而有所差异。然而,竹子独特的生物学特性使得形态分类方法不适用于区分它们。尽管新品种“满协1号”与[原文缺失相关品种名称]相比,在分枝特征和笋箨颜色上有显著差异,但在分子水平上仍缺乏精确的遗传信息。本研究基于[原文缺失相关竹种名称]的全基因组鉴定了231,789个微卫星标记,并详细分析了它们的类型组成和在染色体上的分布。然后,利用TP-M13-SSR荧光标记技术,筛选出34对多态性引物,用于鉴定新品种“满协1号”和[原文缺失相关竹种名称]的11个不同地理来源。我们还为它们构建了DNA指纹图谱。同时,我们将6个多态性SSR定位到[原文缺失相关竹种名称]的基因上,其中SSR673定位到与植物免疫相关的[原文缺失相关基因名称]上。本研究中筛选出的特异性标记可快速鉴定[原文缺失相关竹种名称]的地理来源和新品种,并有助于探索与一些重要性状相关的候选基因。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ff0/11511551/c468ea6a35f6/plants-13-02910-g001.jpg

文献检索

告别复杂PubMed语法,用中文像聊天一样搜索,搜遍4000万医学文献。AI智能推荐,让科研检索更轻松。

立即免费搜索

文件翻译

保留排版,准确专业,支持PDF/Word/PPT等文件格式,支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述,25分钟生成高质量综述,智能提取关键信息,辅助科研写作。

立即免费体验