College of Landscape Architecture, Central South University of Forestry and Technology, Changsha, China.
Nanning University, Nanning, China.
PeerJ. 2024 Apr 19;12:e17275. doi: 10.7717/peerj.17275. eCollection 2024.
Sect. Chang, belonging to the genus, is one of the rare and precious ornamental plants distinguished by a distinctive array of yellow-toned petals. However, the variation mechanisms of petal color in Sect. Chang remains largely unclear.
We conducted an integrated analysis of metabolome and transcriptome to reveal petal coloration mechanism in three species, which have different yellow tones petals, including (CZ, golden yellow), (ZD, light yellow), and (XB, milk white).
A total of 356 flavonoid metabolites were detected, and 295 differential metabolites were screened. The contents of 74 differential metabolites showed an upward trend and 19 metabolites showed a downward trend, among which 11 metabolites were annotated to the KEGG pathway database. We speculated that 10 metabolites were closely related to the deepening of the yellowness. Transcriptome analysis indicated that there were 2,948, 14,018 and 13,366 differentially expressed genes (DEGs) between CZ . ZD, CZ . XB and ZD . XB, respectively. Six key structural genes (, , , , , and ) and five candidate transcription factors (MYB22, MYB28, MYB17, EREBP9, and EREBP13) were involved in the regulation of flavonoid metabolites. The findings indicate that flavonoid compounds influence the color intensity of yellow-toned petals in Sect. Chang. Our results provide a new perspective on the molecular mechanisms underlying flower color variation and present potential candidate genes for Camellia breeding.
节，属，是一种稀有的珍贵观赏植物，以独特的黄色调花瓣为特征。然而，节中花瓣颜色的变异机制仍不清楚。
我们对三个具有不同黄色调花瓣的物种（包括 CZ，金黄色；ZD，浅黄色；XB，乳白色）进行了代谢组学和转录组学的综合分析，以揭示花瓣颜色形成的机制。
共检测到 356 种类黄酮代谢物，筛选出 295 种差异代谢物。74 种差异代谢物的含量呈上升趋势，19 种代谢物呈下降趋势，其中 11 种代谢物被注释到 KEGG 途径数据库中。我们推测 10 种代谢物与黄化加深密切相关。转录组分析表明，在 CZ. ZD、CZ. XB 和 ZD. XB 之间分别有 2948、14018 和 13366 个差异表达基因（DEGs）。六个关键结构基因（、、、、和）和五个候选转录因子（MYB22、MYB28、MYB17、EREBP9 和 EREBP13）参与了类黄酮代谢物的调控。研究结果表明，类黄酮化合物影响了节中黄色调花瓣的颜色强度。我们的研究结果为花颜色变异的分子机制提供了新的视角，并为山茶花的育种提供了潜在的候选基因。
