Liu Jia, Guo Liang, Luo Qiuling, Chen Xiulai, Gao Cong, Song Wei, Liu Liming
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu, China.
School of Pharmaceutical Science, Jiangnan University, Wuxi 214122, Jiangsu, China.
Sheng Wu Gong Cheng Xue Bao. 2021 Apr 25;37(4):1277-1286. doi: 10.13345/j.cjb.200430.
Microbial cell factories capable of producing valuable chemicals from renewable feedstocks provide a promising alternative towards sustainability. However, environmental stress remarkably affects the performance of microbial cell factories. By extending the chronological lifespan of microbial cells, the performance of microbial cell factories can be greatly improved. Firstly, an evaluation system for chronological lifespan and semi-chronological lifespan was established based on the changes in survival rates. Secondly, the addition of anti-aging drugs such as cysteine, carnosine, aminoguanidine and glucosamine increased the chronological lifespan of E. coli by 80%, 80%, 50% and 120%, respectively. Finally, we demonstrated that extending the chronological lifespan of E. coli increased the yield of metabolites produced by E. coli cell factories with endogenous (lactic acid and pyruvic acid) or exogenous (malic acid) metabolic pathway by 30.0%, 25.0%, and 27.0%, respectively. The strategy of extending chronological lifespan of E. coli provides a potential approach for enhancing the performance of microbial cell factories.
能够利用可再生原料生产有价值化学品的微生物细胞工厂为实现可持续发展提供了一种有前景的替代方案。然而，环境压力显著影响微生物细胞工厂的性能。通过延长微生物细胞的寿命，微生物细胞工厂的性能可以得到极大提高。首先，基于存活率的变化建立了细胞寿命和半细胞寿命的评估系统。其次，添加半胱氨酸、肌肽、氨基胍和氨基葡萄糖等抗衰老药物分别使大肠杆菌的细胞寿命延长了80%、80%、50%和120%。最后，我们证明，延长大肠杆菌的细胞寿命分别使具有内源性（乳酸和丙酮酸）或外源性（苹果酸）代谢途径的大肠杆菌细胞工厂产生的代谢物产量提高了30.0%、25.0%和27.0%。延长大肠杆菌细胞寿命的策略为提高微生物细胞工厂的性能提供了一种潜在方法。
