Yu Yong, Zhu Xinna, Bi Changhao, Zhang Xueli
Tianjin Institute of Industrial Biotechnology, Chinese Academy of Science, Tianjin 300308, China.
Sheng Wu Gong Cheng Xue Bao. 2021 May 25;37(5):1564-1577. doi: 10.13345/j.cjb.200716.
As an important model industrial microorganism, Escherichia coli has been widely used in pharmaceutical, chemical industry and agriculture. In the past 30 years, a variety of new strategies and techniques, including artificial intelligence, gene editing, metabolic pathway assembly, and dynamic regulation have been used to design, construct, and optimize E. coli cell factories, which remarkably improved the efficiency for biotechnological production of chemicals. In this review, three key aspects for constructing E. coli cell factories, including pathway design, pathway assembly and regulation, and optimization of global cellular performance, are summarized. The technologies that have played important roles in metabolic engineering of E. coli, as well as their future applications, are discussed.
作为一种重要的模式工业微生物，大肠杆菌已广泛应用于制药、化工和农业领域。在过去30年里，包括人工智能、基因编辑、代谢途径组装和动态调控在内的各种新策略和技术已被用于设计、构建和优化大肠杆菌细胞工厂，这显著提高了化学品生物技术生产的效率。在本综述中，总结了构建大肠杆菌细胞工厂的三个关键方面，包括途径设计、途径组装与调控以及全局细胞性能优化。讨论了在大肠杆菌代谢工程中发挥重要作用的技术及其未来应用。
