同时测定C3H/10T1/2细胞培养物中的抗坏血酸和脱氢抗坏血酸。
Simultaneous determination of ascorbic acid and dehydroascorbic acid in cultures of C3H/10T1/2 cells.
作者信息
Ibric L L, Benedict W F, Peterson A R
机构信息
Department of Pediatrics, Childrens Hospital of Los Angeles, California 90027.
出版信息
In Vitro Cell Dev Biol. 1988 Jul;24(7):669-76. doi: 10.1007/BF02623604.
A reproducible method is described for the separation and quantification of ascorbic acid and dehydroascorbic acid by ion-pairing reverse-phase high performance liquid chromatography and detection by absorbance at 232 nm. Lowest detectable concentrations with a linear response of detection were 5 nmol for ascorbic acid and 50 nmol for dehydroascorbic acid. This method was applied to the analysis of C3H/10T1/2 cells and culture medium after influx or efflux experiments and single or multiple treatments with ascorbic acid. Subsequent measurement of the radioactivity in the eluted fractions increased the detectability of both ascorbic acid and dehydroascorbic acid to 10 to 20 pmol.
描述了一种通过离子对反相高效液相色谱法分离和定量抗坏血酸和脱氢抗坏血酸，并在232nm处通过吸光度检测的可重现方法。检测呈线性响应时，抗坏血酸的最低可检测浓度为5nmol，脱氢抗坏血酸为50nmol。该方法应用于C3H/10T1/2细胞和培养基在摄取或流出实验以及用抗坏血酸进行单次或多次处理后的分析。随后对洗脱组分中的放射性进行测量，将抗坏血酸和脱氢抗坏血酸的可检测性提高到10至20pmol。
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