Chongqing Key Laboratory of Plant Resource Conservation and Germplasm Innovation, SWU-TAAHC Medicinal Plant Joint R&D Centre, School of Life Sciences, Southwest University, Chongqing 400715, China.
Tobacco Breeding and Biotechnology Research Center, Yunnan Academy of Tobacco Agricultural Sciences, Key Laboratory of Tobacco Biotechnological Breeding, National Tobacco Genetic Engineering Research Center, Kunming 650021, China.
ACS Synth Biol. 2021 May 21;10(5):957-963. doi: 10.1021/acssynbio.1c00016. Epub 2021 May 11.
Nootkatone is a valuable sesquiterpene widely used in the food, fragrance, and flavor industries. Its price is very high due to its limited production in grapefruit peels or Alaska cypress heartwoods. Chemical synthesis of nootkatone uses heavy metals, highly flammable compounds, and strong oxidants, which cause severe damage to the environment. In this study, nootkatone is synthesized in , using synthetic biology methods. Engineered coexpressing valencene synthase (VS) and valencene oxidase (VO) in the cytosol produced nootkatone ranging from 0.89 to 8.52 μg/g fresh weight (FW). Furthermore, transgenic coexpressing farnesyl diphosphate synthase (FPS), VS, and VO in plastids produced nootkatone ranging from 12.11 to 47.80 μg/g FW. These results indicated that engineering nootkatone biosynthesis in plastids was superior to that in the cytosol. Meanwhile, artemisinin production was unaltered in nootkatone-producing . Our study developed a green approach for producing nootkatone in with great market potential.
诺卡酮是一种有价值的倍半萜烯，广泛应用于食品、香料和调味行业。由于其在葡萄柚皮或阿拉斯加柏木心材中的产量有限，因此价格非常高。诺卡酮的化学合成使用重金属、高度易燃化合物和强氧化剂，这会对环境造成严重破坏。在这项研究中，使用合成生物学方法在 中合成了诺卡酮。在细胞质中共表达香叶烯合酶（VS）和香叶烯氧化酶（VO）的工程化 产生的诺卡酮量范围为 0.89 至 8.52μg/g 鲜重（FW）。此外，在质体中共表达法呢基二磷酸合酶（FPS）、VS 和 VO 的转基因 产生的诺卡酮量范围为 12.11 至 47.80μg/g FW。这些结果表明，在质体中进行诺卡酮生物合成的工程改造优于在细胞质中进行的工程改造。同时，诺卡酮产生的 中青蒿素的产量保持不变。我们的研究为 中具有巨大市场潜力的诺卡酮生产开发了一种绿色方法。
