Univ Paris Diderot, Sorbonne Paris Cité, Laboratoire Interdisciplinaire des Energies de Demain (LIED), 75205 Paris, France.
Ecologie Systématique Evolution, CNRS, Université Paris-Saclay, AgroParisTech, 91400 Orsay, France.
G3 (Bethesda). 2021 Aug 7;11(8). doi: 10.1093/g3journal/jkab159.
Melanins are pigments used by fungi to withstand various stresses and to strengthen vegetative and reproductive structures. In Sordariales fungi, their biosynthesis starts with a condensation step catalyzed by an evolutionary-conserved polyketide synthase. Here we show that complete inactivation of this enzyme in the model ascomycete Podospora anserina through targeted deletion of the PaPks1 gene results in reduced female fertility, in contrast to a previously analyzed nonsense mutation in the same gene that retains full fertility. We also show the utility of PaPks1 mutants for detecting rare genetic events in P. anserina, such as parasexuality and possible fertilization and/or apomixis of nuclei devoid of mating-type gene.
黑色素是真菌用来抵抗各种压力和增强营养和繁殖结构的色素。在 Sordariales 真菌中，它们的生物合成始于由进化保守的聚酮合酶催化的缩合步骤。在这里，我们通过靶向缺失模型子囊菌 Podospora anserina 中的 PaPks1 基因，证明了该酶的完全失活会导致雌性育性降低，这与之前在同一基因中分析的无意义突变不同，该突变保留了完全的育性。我们还展示了 PaPks1 突变体在检测 P. anserina 中罕见遗传事件（如假配性和可能缺乏交配型基因的核的受精和/或无融合生殖）方面的效用。
