Isolation of sp. nov., a novel bacterium in the family from marine mangrove sediment.

A Gram-stain-negative, strictly aerobic and oval-shaped bacterial strain with a flagellum, designated GS-10, was isolated from mangrove wetland sediment. GS-10 grew at 20-40 °C (optimum, 37 °C), in the pH range of 5.0-11.0 (optimum, 6.0-8.0) and under various NaCl concentrations from 1 to 11 % (w/v) (optimum, 5-6 %). The respiratory quinone was ubiquinone-10, and the predominant polar lipids were phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The major fatty acids (>10 % of the total fatty acids) were summed feature 4 (Ciso I/anteiso B) and summed feature 8 (Cω7 and/or Cω6). The G+C content of the genomic DNA was 63.71 %. On the basis of the results from comparative analysis of the 16S rRNA gene sequence, GS-10 represents a member of the family and had the highest sequence similarity to CECT 4357 (97.47 %), followed by W11-2B (97.03 %), CL-SK44 (96.83 %), CECT 5118 (96.75 %) and CECT 5383 (96.68 %). Phylogenetic trees based on 16S rRNA gene sequences, multilocus sequence analysis (MLSA) and whole genome sequences revealed that GS-10 clustered with species within the genus . The average nucleotide identity (ANI) and the average amino acid identity (AAI) values were calculated from complete genome sequences and indicated that GS-10 represented a novel species of the genus , and the name sp. nov. is proposed for this species. The type strain of is GS-10 (=MCCC 1K03624=KCTC 82131).