Li Dak Sum Yip Yio Chin Kenneth Li Marine Biopharmaceutical Research Center, College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo, 315800, PR China.
Ocean College, Zhejiang University, Zhoushan, 316021, PR China.
Int J Syst Evol Microbiol. 2021 Apr;71(4). doi: 10.1099/ijsem.0.004782.
A Gram-stain-negative, ovoid-shaped, motile and strictly aerobic bacterium, designated ZQ172, was isolated from marine sediment sampled on Zhoushan Island located in the East China Sea. Strain ZQ172 grew optimally at temperatures of 25-30 °C, at NaCl concentration of 2.0 % (w/v) and at pH 7.0-8.5. Catalase and oxidase activities, urease, and nitrate reduction were positive. Indole production, methyl red reaction, HS production and hydrolysis of gelatin, starch, casein and Tweens 20, 40, 60 and 80 were negative. The major cellular fatty acid was summed feature 8 (C 7 and/or C 6). The only respiratory quinone was ubiquinone-10. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and phosphatidylcholine. Comparative analysis of the 16S rRNA gene sequence showed highest similarities to JC2049 (97.5 %), JAMH 043 (97.5 %) and CECT 7735 (97.3 %). Phylogenetic analyses indicated that strain ZQ172 clustered with the genus and was closely related to CECT 7735. The average nucleotide identity and digital DNA-DNA hybridization values between strain ZQ172 and related species of the genus were well below the threshold limit for prokaryotic species delineation. The DNA G+C content was 59.6 mol%. Based on its phenotypic, chemotaxonomic and genotypic data, strain ZQ172 is considered to be a representative of a novel species in the genus , for which the name sp. nov. is proposed. The type strain is ZQ172 (=KCTC 62578=MCCC 1K03531).
一株革兰氏阴性、卵圆形、运动和严格需氧的细菌，被命名为 ZQ172，从中国东海舟山岛的海洋沉积物中分离得到。菌株 ZQ172 在 25-30°C 的温度、2.0%（w/v）的 NaCl 浓度和 pH 值 7.0-8.5 的条件下生长最佳。过氧化氢酶和氧化酶活性、脲酶和硝酸盐还原为阳性。吲哚产生、甲基红反应、HS 产生以及明胶、淀粉、酪蛋白和吐温 20、40、60 和 80 的水解为阴性。主要的细胞脂肪酸是 summed feature 8（C7 和/或 C6）。唯一的呼吸醌是泛醌-10。主要的极性脂类是磷脂酰乙醇胺、磷脂酰甘油、双磷脂酰甘油和磷脂酰胆碱。16S rRNA 基因序列的比较分析显示，与 JC2049（97.5%）、JAMH 043（97.5%）和 CECT 7735（97.3%）的相似度最高。系统发育分析表明，菌株 ZQ172 与属内的其他种聚在一起，与 CECT 7735 关系最为密切。菌株 ZQ172 与属内相关种之间的平均核苷酸同一性和数字 DNA-DNA 杂交值均远低于原核生物种划分的阈值。DNA G+C 含量为 59.6mol%。基于其表型、化学分类和基因型数据，菌株 ZQ172 被认为是属内一个新种的代表，因此建议将其命名为 sp. nov.。模式菌株为 ZQ172（=KCTC 62578=MCCC 1K03531）。
