School of Integrative Engineering, Chung-Ang University, 84 Heukseok-ro, Dongjak-gu, Seoul 06974, Republic of Korea.
School of Integrative Engineering, Chung-Ang University, 84 Heukseok-ro, Dongjak-gu, Seoul 06974, Republic of Korea.
Biotechnol Adv. 2021 Sep-Oct;50:107767. doi: 10.1016/j.biotechadv.2021.107767. Epub 2021 May 8.
To overcome environmental problems caused by the use of fossil resources, microbial cell factories have become a promising technique for the sustainable and eco-friendly development of valuable products from renewable resources. Constructing microbial cell factories with high titers, yields, and productivity requires a balance between growth and production; to this end, tuning gene expression and regulation is necessary to optimise and precisely control complicated metabolic fluxes. In this article, we review the current trends and advances in tuning gene expression and regulation and consider their engineering at each of the three stages of gene regulation: genomic, mRNA, and protein. In particular, the technological approaches utilised in a diverse range of genetic-engineering-based tools for the construction of microbial cell factories are reviewed and representative applications of these strategies are presented. Finally, the prospects for strategies and systems for tuning gene expression and regulation are discussed.
为了克服使用化石资源所带来的环境问题，微生物细胞工厂已成为从可再生资源中可持续且环保地生产有价值产品的一项有前景的技术。构建具有高浓度、高产量和高生产效率的微生物细胞工厂需要在生长和生产之间取得平衡；为此，需要对基因表达和调控进行调谐，以优化和精确控制复杂的代谢通量。在本文中，我们综述了基因表达和调控调谐的最新趋势和进展，并考虑了在基因调控的三个阶段（基因组、mRNA 和蛋白质）对其进行工程化的相关内容。特别地，我们综述了在构建微生物细胞工厂的各种基于遗传工程的工具中所使用的技术方法，并介绍了这些策略的代表性应用。最后，我们讨论了基因表达和调控调谐策略和系统的前景。
