Department of Anatomy and Cell Biology, University of Yamanashi Faculty of Medicine, 1110, Shimo-Kateau, Chuo, Yamanashi 4093898, Japan; Department of Biochemistry and Molecular Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Kita-ku, Okayama 7008525, Japan.
Department of Anatomy and Cell Biology, University of Yamanashi Faculty of Medicine, 1110, Shimo-Kateau, Chuo, Yamanashi 4093898, Japan.
Bone. 2021 Sep;150:116001. doi: 10.1016/j.bone.2021.116001. Epub 2021 May 8.
Primary cilium is a protruding cellular organelle that has various physiological functions, especially in sensory reception. While an avalanche of reports on primary cilia have been published, the function of primary cilia in dental cells remains to be investigated. In this study, we focused on the function of primary cilia in dentin-producing odontoblasts. Odontoblasts, like most other cell types, possess primary cilia, which disappear upon the knockdown of intraflagellar transport protein 88. In cilia-depleted cells, the expression of dentin sialoprotein, an odontoblastic marker, was elevated, while the deposition of minerals was slowed. This was recapitulated by the activation of canonical Wnt pathway, also decreased the ratio of ciliated cells. In dental pulp cells, as they differentiated into odontoblasts, the ratio of ciliated cells was increased, whereas the canonical Wnt signaling activity was repressed. Our results collectively underscore the roles of primary cilia in regulating odontoblastic differentiation through canonical Wnt signaling. This study implies the existence of a feedback loop between primary cilia and the canonical Wnt pathway.
初级纤毛是一种突出的细胞细胞器，具有多种生理功能，特别是在感觉接收中。虽然已经发表了大量关于初级纤毛的报告，但初级纤毛在牙齿细胞中的功能仍有待研究。在这项研究中，我们专注于产生牙本质的成牙本质细胞中的初级纤毛的功能。成牙本质细胞与大多数其他细胞类型一样，具有初级纤毛，这些纤毛在内鞭毛运输蛋白 88 敲低时消失。在纤毛缺失的细胞中，牙本质涎蛋白的表达升高，而矿物质的沉积速度减慢。这是通过经典 Wnt 途径的激活来重现的，也降低了有纤毛细胞的比例。在牙髓细胞中，当它们分化为成牙本质细胞时，有纤毛细胞的比例增加，而经典 Wnt 信号活性受到抑制。我们的研究结果共同强调了初级纤毛通过经典 Wnt 信号调节成牙本质细胞分化的作用。这项研究表明初级纤毛和经典 Wnt 途径之间存在反馈回路。
