Guang Zhou Institute for Food Inspection, Guangzhou 511410, China.
Guang Zhou Institute for Food Inspection, Guangzhou 511410, China.
Food Chem. 2021 Oct 30;360:129948. doi: 10.1016/j.foodchem.2021.129948. Epub 2021 Apr 24.
A LC-Q-Orbitrap HRMS analytical method for both qualitative screening and quantitative determination of 90 synthetic dyes including ten groups of isomers in foods has been established. An in-house synthetic dyes database and characteristic ions were also developed. Based on Q-Orbitrap HRMS, mass spectrum and fragmentation patterns of synthetic dyes were studied, which indicated that double charged ions were usually the main precursor ions. Matrix effects were successfully eliminated by the C d-SPE clean-up coupled with dilute and shoot approach with methanol-water (1:4, v/v) in 100-fold. For most of the compounds, mean recoveries were satisfactory between 70% and 120% with RSD < 20% at three spiked level in the range of 0.025-1.0 mg/kg. The screening detection limits ranged from 0.025 - 1.0 mg/kg. Method validation showed that the established method was efficient, rapid and high-throughput, which has been successfully applied to the monitoring of these water-soluble synthetic dyes in foods.
建立了一种 LC-Q-Orbitrap HRMS 分析方法，用于定性筛选和定量测定食品中 90 种合成染料（包括十组异构体）。还开发了内部合成染料数据库和特征离子。基于 Q-Orbitrap HRMS，研究了合成染料的质谱和碎片模式，结果表明双电荷离子通常是主要的前体离子。通过 C d-SPE 净化结合甲醇-水（1:4，v/v）在 100 倍稀释和进样方法，成功消除了基质效应。对于大多数化合物，在 0.025-1.0 mg/kg 的三个加标水平下，回收率在 70%-120%之间，相对标准偏差（RSD）<20%。筛选检测限范围为 0.025-1.0 mg/kg。方法验证表明，该方法高效、快速、高通量，已成功应用于食品中这些水溶性合成染料的监测。
